Xu Duoxia, Yuan Fang, Gao Yanxiang, Panya Atikorn, McClements David Julian, Decker Eric Andrew
College of Food Science & Nutritional Engineering, Beijing Key Laboratory of Functional Food from Plant Resources, China Agricultural University, Beijing, China; Department of Food Science, University of Massachusetts, Amherst, MA, USA; School of Food & Chemical Engineering, Beijing Technology & Business University, Beijing, China.
College of Food Science & Nutritional Engineering, Beijing Key Laboratory of Functional Food from Plant Resources, China Agricultural University, Beijing, China.
Food Chem. 2014 Aug 1;156:374-9. doi: 10.1016/j.foodchem.2014.02.019. Epub 2014 Feb 13.
The impact of a whey protein isolate (WPI)-beet pectin (BP) conjugate (formed by dry-heating) on the physical properties and digestibilities of β-carotene and carrier oil in oil-in-water emulsions was studied when they passed through a model gastrointestinal system. β-Carotene emulsions were stabilized by WPI, unconjugated and conjugated WPI-BP, separately. The emulsions were then passed through an in vitro digestion model and the mean droplet size, droplet distribution, zeta-potential, free fatty acids and β-carotene released were measured. The stability to droplet flocculation and coalescence during digestion was increased for the WPI-BP conjugate stabilized emulsion. Addition of BP onto the WPI stabilized emulsions could inhibit the releases of carrier oil (MCT) and β-carotene. The releases of free fatty acids and β-carotene did not differ greatly between the unconjugated and conjugated WPI-BP stabilized emulsions. These results have important implications for protein-polysaccharide stabilized emulsions and conjugates used for the protection and delivery of bioactive compounds.
研究了乳清分离蛋白（WPI）-甜菜果胶（BP）共轭物（通过干热形成）对水包油乳液中β-胡萝卜素和载体油在通过模拟胃肠系统时的物理性质和消化率的影响。β-胡萝卜素乳液分别由WPI、未共轭和共轭的WPI-BP稳定。然后将乳液通过体外消化模型，测量平均液滴大小、液滴分布、ζ电位、游离脂肪酸和释放的β-胡萝卜素。对于WPI-BP共轭物稳定的乳液，消化过程中对液滴絮凝和聚结的稳定性增加。在WPI稳定的乳液中添加BP可以抑制载体油（中链甘油三酯）和β-胡萝卜素的释放。未共轭和共轭的WPI-BP稳定乳液之间游离脂肪酸和β-胡萝卜素的释放没有太大差异。这些结果对用于生物活性化合物保护和递送的蛋白质-多糖稳定乳液和共轭物具有重要意义。
