Zhang Wenguan, Hao Jia, Yuan Yanan, Xu Duoxia
Beijing Key Laboratory of Flavor Chemistry, Beijing Laboratory for Food Quality and Safety, Beijing Advanced Innovation Center for Food Nutrition and Human Health (BTBU), Beijing Engineering and Technology Research Center of Food Additives, School of Food and Health, Beijing Higher Institution Engineering Research Center of Food Additives and Ingredients, Beijing Technology and Business University, Beijing, China.
Front Nutr. 2022 Apr 11;9:878725. doi: 10.3389/fnut.2022.878725. eCollection 2022.
In this study, carboxymethyl cellulose (CMC) was added to soybean protein isolate (SPI)-stabilized rice bran oil (RBO) emulsion to improve its physicochemical stability and free fatty acid (FFA) release characteristics. RBO emulsions stabilized by SPI and various contents of CMC were prepared and assessed by measuring zeta potential, particle size, transmission, and microstructure, the rheological properties were analyzed by dynamic shear rheometer. In addition, its chemical stability was characterized by a storage experiment, and the FFA release was explored by a simulated gastrointestinal tract (GIT) model. It showed that the negative charge of the droplets of RBO emulsion was increased with increasing CMC content. The decrease in transmission of SPI-stabilized RBO emulsion with increasing CMC content was due to the droplets not being free to move by the special network interaction and an increase in the viscosity. According to the determination of the reactive substances of lipid hydroperoxide and thiobarbituric acid during 30 days storage at 37°C, the chemical stability of the emulsion added with CMC was enhanced compared with the SPI-stabilized RBO emulsion. digestion studies not only evaluated the structural changes of RBO emulsions at different stages, but also found that RBO emulsion with CMC showed a higher level of free fatty acids release in comparison with that without CMC. It indicated that the utilization of CMC can improve the bioavailability of RBO emulsions.
在本研究中,将羧甲基纤维素(CMC)添加到大豆分离蛋白(SPI)稳定的米糠油(RBO)乳液中,以改善其物理化学稳定性和游离脂肪酸(FFA)释放特性。制备了由SPI和不同含量的CMC稳定的RBO乳液,并通过测量zeta电位、粒径、透光率和微观结构进行评估,用动态剪切流变仪分析其流变特性。此外,通过储存实验表征其化学稳定性,并通过模拟胃肠道(GIT)模型探究FFA释放情况。结果表明,随着CMC含量的增加,RBO乳液液滴的负电荷增加。随着CMC含量的增加,SPI稳定的RBO乳液透光率降低,这是由于特殊的网络相互作用使液滴无法自由移动以及粘度增加所致。根据在37°C储存30天期间对脂质过氧化氢和硫代巴比妥酸反应性物质的测定,与SPI稳定的RBO乳液相比,添加CMC的乳液化学稳定性增强。消化研究不仅评估了RBO乳液在不同阶段的结构变化,还发现与未添加CMC的RBO乳液相比,添加CMC的RBO乳液释放出更高水平的游离脂肪酸。这表明利用CMC可以提高RBO乳液的生物利用度。