Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei, Taiwan; Department of Otolaryngology, National Taiwan University Hospital and National Taiwan University, College of Medicine, Taipei, Taiwan.
Department of Otolaryngology, National Taiwan University Hospital and National Taiwan University, College of Medicine, Taipei, Taiwan.
J Formos Med Assoc. 2014 Mar;113(3):173-8. doi: 10.1016/j.jfma.2012.05.006. Epub 2012 Oct 12.
BACKGROUND/PURPOSE: Different photosensitizer-mediated photodynamic therapy (PDT) has different intracellular cytotoxic cascades. Previous reports showed that 5-aminolevulinic acid (5-ALA)-mediated PDT suppressed the migration and invasion of head and neck cancer cells. Unlike from 5-ALA, which mainly targets the mitochondria of cells, meta-tetrahydroxyphenyl chlorin (m-THPC) mainly accumulates in the endoplasmic reticulum and Golgi complex. Does m-THPC PDT inhibit the migration and invasion of cancer cells?
The effect of m-THPC PDT with a sublethal dose sufficient to kill around 20% of cells on the migration and invasion of a nasopharyngeal carcinoma KJ-1 cell line was studied by wound healing and Matrigel invasion assays.
In the wound healing assay, the migration distance of KJ-1 cells decreased significantly from 0.71 ± 0.02 mm in the control cells to 0.31 ± 0.03 mm in the PDT-treated cells 24 hours after light treatment (p < 0.05) and the migration distance also decreased significantly from 1.02 ± 0.07 mm in the control cells to 0.32 ± 0.04 mm in the PDT-treated cells 48 hours after treatment (p < 0.05). In the Matrigel invasion assay, the number of the invaded KJ-1 cells in PDT treated group was also statistically significantly less than those without PDT treatment (p < 0.05).
This study demonstrates that a sublethal dose of m-THPC PDT inhibits the migration and invasion of nasopharyngeal carcinoma cells in vitro.
背景/目的：不同的光敏剂介导的光动力疗法（PDT）具有不同的细胞内细胞毒性级联。以前的报告表明，5-氨基酮戊酸（5-ALA）介导的 PDT 抑制了头颈部癌细胞的迁移和侵袭。与主要靶向细胞线粒体的 5-ALA 不同，间四羟基苯并卟啉（m-THPC）主要积聚在内质网和高尔基体复合物中。m-THPC-PDT 是否抑制癌细胞的迁移和侵袭？
通过划痕愈合和 Matrigel 侵袭实验研究了亚致死剂量的 m-THPC-PDT 对鼻咽癌细胞系 KJ-1 细胞迁移和侵袭的影响。
在划痕愈合实验中，与对照组（0.71 ± 0.02mm）相比，光处理 24 小时后 PDT 处理组 KJ-1 细胞的迁移距离显著减少（0.31 ± 0.03mm，p < 0.05），且在处理 48 小时后 PDT 处理组的迁移距离也显著减少（0.32 ± 0.04mm，p < 0.05）。在 Matrigel 侵袭实验中，PDT 处理组中侵袭的 KJ-1 细胞数量也明显少于未进行 PDT 处理的细胞（p < 0.05）。
本研究表明，亚致死剂量的 m-THPC-PDT 抑制了体外鼻咽癌细胞的迁移和侵袭。
