State Key Laboratory of Microbial Metabolism and School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
School of Environmental Science & Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.
Chemosphere. 2014 Aug;108:411-7. doi: 10.1016/j.chemosphere.2014.02.042. Epub 2014 Mar 13.
Fullerene (C60) is a nanoparticle that has been widely studied and applied in numerous commodities. However, there are concerns regarding its potential negative impact on the environment. A fullerene colloidal suspension (nC60) is known for its property of selectively inhibiting the growth of microorganisms. In this study, using denaturing gradient gel electrophoresis fingerprinting technology, we found that fullerene altered the structure of a sludge-derived microbial community. Specifically, the bacteria from Bacillus, Acidovorax and Cloacibacterium genera were enriched in abundance when supplemented with nC60 at pH 6.5 under aerobic conditions. The effects of the fullerene colloidal suspension on a strain of Bacillus isolated from the same microbial community were evaluated to further characterize the growth-stimulating effect of nC60. The biomass of cultures of this strain incubated with nC60 concentrations ranging from 3 mg L(-1) to 7 mg L(-1) was approximately twice that of the control during the stationary phase. The fullerene also induced higher superoxide dismutase activity in Bacillus cereus. Furthermore, the nitrate removal rate of B. cereus increased to nearly 55% in the presence of 5 mg L(-1) nC60, compared to 35% for the control. Meanwhile, the cumulative loading amount of nitrite was reduced from 33 μg mL(-1) to 25 μg mL(-1) by the addition of 5 mg L(-1) nC60. Our results demonstrate that the fullerene colloidal suspension is conditionally capable of promoting the growth and denitrification metabolism of certain bacteria, such as B. cereus. Fullerene might have both inhibitory and stimulatory effects on microorganisms in various environments.
富勒烯（C60）是一种已被广泛研究和应用于众多商品的纳米颗粒。然而，人们对其潜在的环境负面影响表示担忧。富勒烯胶态悬浮液（nC60）以选择性抑制微生物生长的特性而闻名。在这项研究中，我们使用变性梯度凝胶电泳指纹图谱技术发现，富勒烯改变了污泥衍生微生物群落的结构。具体而言，在 pH 值为 6.5 且有氧条件下，当补充 nC60 时，来自芽孢杆菌属、食酸菌属和产碱杆菌属的细菌在丰度上得到了富集。还评估了从同一微生物群落中分离出的一株芽孢杆菌对富勒烯胶态悬浮液的影响，以进一步表征 nC60 的促生长作用。与对照组相比，在含 3mg/L 至 7mg/L nC60 的培养物中，该菌株的培养物生物量在静止期约增加了一倍。富勒烯还诱导了更高的枯草芽孢杆菌超氧化物歧化酶活性。此外，在 5mg/L nC60 的存在下，枯草芽孢杆菌的硝酸盐去除率从 35%增加到近 55%，而对照组为 35%。同时，通过添加 5mg/L nC60，亚硝酸根的累积负载量从 33μg/mL 减少到 25μg/mL。我们的结果表明，富勒烯胶态悬浮液有条件地能够促进某些细菌（如枯草芽孢杆菌）的生长和反硝化代谢。在不同的环境中，富勒烯可能对微生物既有抑制作用又有刺激作用。
