Department of Chemical Engineering, University of Alicante, Carretera de San Vicente S/N, 03690 Alicante, Spain.
Department of Chemical Engineering, University of Alicante, Carretera de San Vicente S/N, 03690 Alicante, Spain.
Chemosphere. 2014 Sep;110:129-35. doi: 10.1016/j.chemosphere.2014.02.029. Epub 2014 Mar 13.
Polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans (PCDD/F) formation was studied, in vitro, with two different chlorophenol mixtures (group "di+tri" 2,4-dichlorophenol; 2,3,4-, 2,3,5-, and 3,4,5-trichlorophenols and group "tri+tetra+penta" with 2,4,5-trichlorophenol, 2,3,4,6-tetrachlorophenol and pentachlorophenol) and two different lignolytic enzymes, lignin and manganese peroxidase (LiP and MnP respectively), which can be found during the composting process of sewage sludge. The concentrations of PCDD/F in final samples are compared to the PCDD/F content of the control samples containing the chlorophenols. High increases were observed for experiments with MnP and phosphate buffer. Experiments that contained tri-, tetra- and pentachlorophenol with MnP resulted in more than 8·10(8)ng of OCDD kg(-1) chlorophenol which was much higher than the initial amount (1·10(7)ng OCDD kg(-1) chlorophenol). In relation to LiP experiments, only those at 37°C showed a moderate increase (from 1.3·10(7) to 2.6·10(7)ng of OCDD kg(-1) chlorophenol). The results agree with the literature in which high amounts of HpCDD and OCDD were found after a composting process and could explain the biogenic formation suggested by others, but the incidence on the total toxicity is less than that expected.
多氯二苯并对二恶英和多氯二苯并呋喃（PCDD/F）的形成进行了研究，采用两种不同的氯苯酚混合物（“二+三”组 2,4-二氯苯酚；2,3,4-，2,3,5-和 3,4,5-三氯苯酚和“三+四+五”组 2,4,5-三氯苯酚、2,3,4,6-四氯苯酚和五氯苯酚）和两种不同的木质素降解酶（木质素和锰过氧化物酶（LiP 和 MnP））进行体外研究，这些酶可在污水污泥堆肥过程中找到。最终样品中的 PCDD/F 浓度与含有氯苯酚的对照样品中的 PCDD/F 含量进行了比较。在 MnP 和磷酸盐缓冲液存在的情况下进行的实验中，观察到高浓度的 PCDD/F。含有三氯、四氯和五氯苯酚的 MnP 实验导致 OCDD 含量超过 8·10(8)ng 氯苯酚-1kg(-1)，远高于初始量（1·10(7)ng OCDD kg(-1)氯苯酚）。与 LiP 实验相比，只有在 37°C 下的实验显示出适度增加（从 1.3·10(7)增加到 2.6·10(7)ng OCDD kg(-1)氯苯酚）。这些结果与文献一致，在堆肥过程后发现大量的 HpCDD 和 OCDD，这可以解释其他人提出的生物成因，但对总毒性的影响小于预期。
