Wittsiepe J, Kullmann Y, Schrey P, Selenka F, Wilhelm M
Ruhr-Universität Bochum, Abteilung für Hygiene, Sozial- und Umweltmedizin, Germany.
Toxicol Lett. 1999 Jun 1;106(2-3):191-200. doi: 10.1016/s0378-4274(99)00066-1.
Chlorophenols (CP) are transformed in vitro to polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/F) by a peroxidase-catalyzed oxidation. This is shown for 2,4,5-tri-, 2,3,4,6-tetra- and pentachlorophenol with plant horseradish peroxidase and with myeloperoxidase recovered from human leukocytes, each in the presence of hydrogen peroxide. The yield, the reaction and the PCDD/F-pattern found are dependent on the CP. The amounts of PCDD/F formed within 4 or 24 h are in the micromol/mol-range for all substrates and both peroxidases. The experiments suggest that biochemical formation of PCDD/F from precursors such as CPs can take place in the human body and that this metabolic pathway may lead to a higher inner exposure to PCDD/F than up to now assumed based on intake data for PCDD/F.
氯酚（CP）在体外通过过氧化物酶催化氧化转化为多氯代二苯并 - 对 - 二恶英和二苯并呋喃（PCDD/F）。以辣根过氧化物酶和从人白细胞中提取的髓过氧化物酶分别在过氧化氢存在的情况下，对2,4,5 - 三氯酚、2,3,4,6 - 四氯酚和五氯酚进行了实验，结果表明了上述转化过程。所得到的产率、反应情况以及PCDD/F模式均取决于氯酚。对于所有底物和两种过氧化物酶，在4小时或24小时内形成的PCDD/F量均在微摩尔/摩尔范围内。这些实验表明，人体内可能会发生从CP等前体物质生化形成PCDD/F的过程，并且这种代谢途径可能导致人体内部对PCDD/F的暴露程度比目前基于PCDD/F摄入数据所假设的更高。
