Department of Biochemistry, Shivaji University, Kolhapur 416 004, India.
Department of Biology, Hong Kong Baptist University, Kowloon, Hong Kong.
Chemosphere. 2014 Aug;109:234-8. doi: 10.1016/j.chemosphere.2014.02.009. Epub 2014 Mar 12.
Galactomyces geotrichum MTCC 1360 exhibited 86% decolorization of azo dye Reactive Yellow-84A (50mgL(-1)) within 30h at 30°C and pH 7.0 under static condition. Examination of azoreductase, laccase and tyrosinase enzyme activities confirmed their prominent role in Reactive Yellow-84A degradation. Considerable reduction of COD (73%) and TOC (62%) during degradation of the dye was indicative of conversion of complex dye into simple products, which were further analyzed by HPLC, FTIR, GC-MS and HPTLC. The degradation products were identified as 4(5-hydroxy, 4-amino cyclopentane) sulfobenzene and 4(5-hydroxy cyclopentane) sulfobenzene by GC-MS. In addition, when G. geotrichum was applied to decolorize textile effluent, it showed 85% of true color removal (ADMI removal) within 72h, along with a significant reduction in TOC and COD. Phytotoxicity studies revealed the less toxic nature of degraded Reactive Yellow-84A as compared to original dye.
球形节酵母 MTCC 1360 在 30°C 和 pH 值 7.0 条件下，在静态条件下，30h 内对 50mg/L 的偶氮染料活性艳黄-84A 展现了 86%的脱色率。通过检测偶氮还原酶、漆酶和酪氨酸酶的活性，证实了它们在活性艳黄-84A 降解中具有重要作用。在染料降解过程中，COD（73%）和TOC（62%）的大量减少表明复杂染料被转化为简单产物，这些产物进一步通过 HPLC、FTIR、GC-MS 和 HPTLC 进行了分析。GC-MS 鉴定降解产物为 4(5-羟基，4-氨基环戊烷)亚磺基苯和 4(5-羟基环戊烷)亚磺基苯。此外，当球形节酵母用于脱色纺织废水时，在 72h 内实现了 85%的真实颜色去除（ADMI 去除），同时 TOC 和 COD 也显著降低。植物毒性研究表明，与原始染料相比，降解后的活性艳黄-84A 的毒性较低。
