Amin Shivani, Rastogi Rajesh Prasad, Chaubey Mukesh Ghanshyam, Jain Kunal, Divecha Jyoti, Desai Chirayu, Madamwar Datta
Post-Graduate Department of Biosciences, UGC-Centre of Advanced Study, Sardar Patel University, Satellite Campus, Bakrol, India.
Department of Biotechnology, Shree A. N. Patel PG Institute of Science and Research, Sardar Patel University, Anand, India.
Front Microbiol. 2020 Sep 24;11:576680. doi: 10.3389/fmicb.2020.576680. eCollection 2020.
An efficient diazo dye degrading bacterial strain, sp. DMS2 was isolated from a long-term textile dye polluted environment. The strain was assessed for its innate ability to completely degrade and detoxify Direct Red 81 (DR81) textile dye under microaerophilic conditions. The degradation ability of strain showed significant results on optimizing the nutritional and environmental parameters. Based on statistical models, maximum efficiency of decolorization achieved within 24 h for 100 mg/l of dye supplemented with glucose (0.02%), MgSO (0.002%) and urea (0.5%) at 30°C and pH (7.0). Moreover, a significant catabolic induction of a laccase and azoreductase suggested its vital role in degrading DR81 into three distinct metabolites (intermediates) as by-products. Further, toxicity analysis of intermediates were performed using seeds of common edible plants, aquatic plant (phytotoxicity) and the nematode model (animal toxicity), which confirmed the non-toxic nature of intermediates. Thus, the inclusive study of DMS2 showed promising efficiency in bioremediation approach for treating industrial effluents.
从长期受纺织染料污染的环境中分离出一株高效降解重氮染料的细菌菌株，即DMS2菌。评估了该菌株在微需氧条件下完全降解和解毒直接红81（DR81）纺织染料的内在能力。菌株的降解能力在优化营养和环境参数方面显示出显著效果。基于统计模型，在30°C和pH值为7.0的条件下，对于添加了葡萄糖（0.02%）、硫酸镁（0.002%）和尿素（0.5%）的100 mg/l染料，24小时内实现了最大脱色效率。此外，漆酶和偶氮还原酶的显著分解代谢诱导表明其在将DR81降解为三种不同代谢产物（中间体）作为副产物方面的重要作用。进一步地，使用常见食用植物种子、水生植物（植物毒性）和线虫模型（动物毒性）对中间体进行了毒性分析，证实了中间体的无毒性质。因此，对DMS2的全面研究表明其在生物修复处理工业废水方面具有良好的效率。
