Liu Wenqiang, Yin Jiqing, Kou Xiaochen, Jiang Yonghua, Gao Haibo, Zhao Yanhong, Huang Bo, He Wenteng, Wang Hong, Han Zhiming, Gao Shaorong
College of Biological Sciences, China Agricultural University, Beijing 100094, China; National Institute of Biological Sciences, NIBS, Beijing 102206, China; School of Life Sciences and Technology, Tongji University, Shanghai 200092, China.
National Institute of Biological Sciences, NIBS, Beijing 102206, China; School of Life Sciences and Technology, Tongji University, Shanghai 200092, China.
Cell Rep. 2014 Mar 27;6(6):1008-1016. doi: 10.1016/j.celrep.2014.02.018. Epub 2014 Mar 13.
It has been demonstrated that reprogramming factors are sequestered in the pronuclei of zygotes after fertilization, because zygotes enucleated at the M phase instead of interphase of the first mitosis can support the development of cloned embryos. However, the contribution of the parental pronucleus derived from either the sperm or the oocyte in reprogramming remains elusive. Here, we demonstrate that the parental pronuclei have asymmetric reprogramming capacities and that the reprogramming factors reside predominantly in the male pronucleus. As a result, only female pronucleus-depleted (FPD) mouse zygotes can reprogram somatic cells to a pluripotent state and support the full-term development of cloned embryos; male pronucleus-depleted (MPD) zygotes fail to support somatic cell reprogramming. We further demonstrate that fusion of an additional male pronucleus into a zygote greatly enhances reprogramming efficiency. Our data provide a clue to further identify critical reprogramming factors in the male pronucleus.
研究表明，重编程因子在受精后被隔离于合子的原核中，因为在第一次有丝分裂的M期而非间期去核的合子能够支持克隆胚胎的发育。然而，来自精子或卵母细胞的亲代原核在重编程中的作用仍不清楚。在此，我们证明亲代原核具有不对称的重编程能力，且重编程因子主要存在于雄原核中。因此，只有去除雌原核（FPD）的小鼠合子能够将体细胞重编程为多能状态并支持克隆胚胎的足月发育；去除雄原核（MPD）的合子则无法支持体细胞重编程。我们进一步证明，向合子中额外融合一个雄原核可大大提高重编程效率。我们的数据为进一步鉴定雄原核中的关键重编程因子提供了线索。
