Zhang Bailin, Morales Andres W, Peterson Ralph, Tang Liang, Ye Jing Yong
Department of Biomedical Engineering, University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USA.
Department of Biomedical Engineering, University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USA.
Biosens Bioelectron. 2014 Aug 15;58:107-13. doi: 10.1016/j.bios.2014.02.057. Epub 2014 Feb 28.
A biosensor has been developed with a photonic crystal structure used in a total-internal-reflection (PC-TIR) configuration for label-free detection of a cardiac biomarker: Troponin I (cTnI). In contrast to a conventional optical microcavity that has a closed structure with its cavity layer sandwiched between two high-reflection surfaces, the PC-TIR configuration creates a unique open microcavity, which allows its cavity layer (sensing layer) to be easily functionalized and directly exposed to analyte molecules for bioassays. In this study, a PC-TIR sensor has been used for the label-free measurements of cardiac biomarkers by monitoring the changes in the resonant condition of the cavity due to biomolecular binding processes. Antibodies against cTnI are immobilized on the sensor surface for specific detection of cTnI with a wide range of concentrations. Detection limit of cTnI with a concentration as low as 0.1ngmL(-1) has been achieved.
已开发出一种生物传感器，其具有用于全内反射（PC-TIR）配置的光子晶体结构，用于无标记检测心脏生物标志物：肌钙蛋白I（cTnI）。与传统光学微腔不同，传统光学微腔具有封闭结构，其腔层夹在两个高反射表面之间，而PC-TIR配置创建了一个独特的开放微腔，这使得其腔层（传感层）能够轻松功能化并直接暴露于分析物分子以进行生物测定。在本研究中，通过监测由于生物分子结合过程导致的腔谐振条件变化，PC-TIR传感器已用于心脏生物标志物的无标记测量。抗cTnI抗体固定在传感器表面，用于特异性检测各种浓度的cTnI。已实现低至0.1ngmL(-1)浓度的cTnI的检测限。
