Zhu Yuqin, Duan Zilei, Mo Guoxiang, Shen Chuanbin, Lv Longbao, Chen Wenlin, Lai Ren
Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China; Life Sciences College of Nanjing Agricultural University, Nanjing 210095, Jiangsu, China.
Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China; Kunming College of Life Science, University of Chinese Academy of Sciences, Beijing 100009, China.
Biochimie. 2014 Jul;102:112-6. doi: 10.1016/j.biochi.2014.02.014. Epub 2014 Mar 12.
26RFa is one of neuroendocrine peptide groups in the RFamide peptide family containing conserved Arg-Phe/Tyr-NH2 motif at their C-terminus. They exert multiple biological functions in vertebrates. A novel 26RFa peptide (TC26RFa) with unique structure is identified from the tree shrew of Tupaia belangeri chinensis in the present study. In structure, different from other 26RFa peptides containing conserved Phe-Arg-Phe-NH2 motif at their C-terminus, there is a Phe-Arg-Tyr-NH2 C-terminus in TC26RFa. It has been found that TC26RFa of intraperitoneal injection exerts strong analgesic activities in several mice models including acetic acid-induced abdominal writhing, formalin-induced paw licking, and thermal pain-induced tail withdrawal. It shows comparable analgesic ability with morphine. In addition, this peptide has been found to inhibit inflammatory factor secretion (including tumor necrosis factor-α, interleukin-6, and interleukin-1β) induced by lipopolysaccharides (LPS). Furthermore, it stimulates secretion of the anti-inflammatory factor, interleukin-10. In addition to the identification of a novel 26RFa peptide from tree shrew, a new type of function (anti-inflammation) involved in 26RFa peptide is discovered.
26RFa是RFamide肽家族中的神经内分泌肽组之一，在其C末端含有保守的Arg-Phe/Tyr-NH2基序。它们在脊椎动物中发挥多种生物学功能。在本研究中，从中国树鼩（Tupaia belangeri chinensis）中鉴定出一种具有独特结构的新型26RFa肽（TC26RFa）。在结构上，与其他在C末端含有保守的Phe-Arg-Phe-NH2基序的26RFa肽不同，TC26RFa的C末端是Phe-Arg-Tyr-NH2。研究发现，腹腔注射的TC26RFa在几种小鼠模型中发挥强大的镇痛活性，包括醋酸诱导的扭体反应、福尔马林诱导的舔足反应和热痛诱导的甩尾反应。它显示出与吗啡相当的镇痛能力。此外，已发现该肽可抑制脂多糖（LPS）诱导的炎症因子分泌（包括肿瘤坏死因子-α、白细胞介素-6和白细胞介素-1β）。此外，它还刺激抗炎因子白细胞介素-10的分泌。除了从树鼩中鉴定出一种新型26RFa肽外，还发现了26RFa肽涉及的一种新功能（抗炎）。
