comTAR：一个用于预测和描述植物保守 miRNA 靶标的网络工具。
comTAR: a web tool for the prediction and characterization of conserved microRNA targets in plants.
机构信息
IBR (Instituto de Biología Molecular y Celular de Rosario), CONICET and Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, 2000 Rosario, Argentina.
出版信息
Bioinformatics. 2014 Jul 15;30(14):2066-7. doi: 10.1093/bioinformatics/btu147. Epub 2014 Mar 14.
MOTIVATION
MicroRNAs (miRNAs) are major regulators of gene expression in plants and animals. They recognize their target messenger RNAs (mRNAs) by sequence complementarity and guide them to cleavage or translational arrest. So far, the prediction of plant miRNA-target pairs generally relies on the use of empirical parameters deduced from known miRNA-target interactions.
RESULTS
We developed comTAR, a web tool for the prediction of miRNA targets that is mainly based on the conservation of the potential regulation in different species. We used data generated from a pipeline applied to transcript datasets of 33 angiosperms that was used to build a database of potential miRNA targets of different plant species. The database contains information describing each miRNA-target pair, their function and evolutionary conservation, while the results are displayed in a user-friendly interface. The tool also allows the search using new miRNAs.
AVAILABILITY AND IMPLEMENTATION
The Web site is free to all users, with no login requirements, at http://rnabiology.ibr-conicet.gov.ar/comtar.
动机
MicroRNAs（miRNAs）是动植物基因表达的主要调控因子。它们通过序列互补识别其靶信使 RNA（mRNA），并引导它们进行切割或翻译抑制。到目前为止，植物 miRNA-靶对的预测通常依赖于使用从已知 miRNA-靶相互作用中推导出来的经验参数。
结果
我们开发了 comTAR，这是一个用于 miRNA 靶预测的网络工具，主要基于不同物种中潜在调控的保守性。我们使用从应用于 33 种被子植物转录数据集的管道生成的数据，用于构建不同植物物种潜在 miRNA 靶的数据库。该数据库包含描述每个 miRNA-靶对的信息，以及它们的功能和进化保守性，而结果则显示在用户友好的界面中。该工具还允许使用新的 miRNAs 进行搜索。
可用性和实现
该网站对所有用户免费开放，无需登录要求，网址为 http://rnabiology.ibr-conicet.gov.ar/comtar。
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