在切除的胰腺导管腺癌中，高神经纤毛蛋白1表达与血管生成及总体生存率低相关。

High neuropilin 1 expression was associated with angiogenesis and poor overall survival in resected pancreatic ductal adenocarcinoma.

作者信息

Ben Qiwen, Zheng Jianming, Fei Jian, An Wei, Li Ping, Li Zhaoshen, Yuan Yaozong

机构信息

From the *Department of Gastroenterology, Ruijin Hospital, Shanghai Jiaotong University; †Department of Pathology, Changhai Hospital of Second Military Medical University; ‡Department of General Surgery, Ruijin Hospital, Shanghai Jiaotong University; and §Department of Gastroenterology, Changhai Hospital of Second Military Medical University, Shanghai, China.

出版信息

Pancreas. 2014 Jul;43(5):744-9. doi: 10.1097/MPA.0000000000000117.

DOI:10.1097/MPA.0000000000000117

PMID:24632553

Abstract

OBJECTIVES

Neuropilin 1 (NRP-1) appears to promote angiogenesis by acting as a coreceptor with vascular endothelial growth factor receptor. We correlated NRP-1 expression with microvessel density (MVD) and overall survival (OS) in human pancreatic ductal adenocarcinomas (PDACs).

METHODS

Neuropilin 1 expression was graded semiquantitatively using immunohistochemistry in patients with resected PDAC. Moreover, MVD was determined with an anti-CD31 antibody staining. Expression of NRP-1 was correlated with MVD and clinicopathologic features in patients with PDAC. Overall survival effects of NRP-1 expression were evaluated by multivariate Cox regression and Kaplan-Meier analyses.

RESULTS

High NRP-1 expression was associated with advanced Union for International Cancer Control stage (P = 0.046), T stage (P = 0.031), and lymph node invasion (P = 0.045). Microvessel density was significantly higher in the tumors with high NRP-1 expression than that in the tumors with low NRP-1 expression (mean, 13.9 [SD, 9.1] vs 10.2 [SD, 7.2] per high-power field; P = 0.001). The multivariate Cox regression analysis demonstrated that high NRP-1 expression was independently associated with reduced OS (hazard ratio, 2.10; 95% confidence interval, 1.19-3.70).

CONCLUSIONS

Neuropilin 1 is highly expressed in PDACs, and high expression of NRP-1 is significantly correlated with angiogenesis, advanced tumor-node-metastasis stage, p T stage, node invasion, and poor postoperative OS.

摘要

目的

神经纤毛蛋白1（NRP - 1）似乎通过作为血管内皮生长因子受体的共受体来促进血管生成。我们将人胰腺导管腺癌（PDAC）中NRP - 1的表达与微血管密度（MVD）及总生存期（OS）进行了相关性分析。

方法

对接受手术切除的PDAC患者，采用免疫组织化学方法对神经纤毛蛋白1的表达进行半定量分级。此外，用抗CD31抗体染色来测定MVD。将PDAC患者中NRP - 1的表达与MVD及临床病理特征进行相关性分析。通过多因素Cox回归分析和Kaplan - Meier分析评估NRP - 1表达对总生存期的影响。

结果

NRP - 1高表达与国际癌症控制联盟晚期（P = 0.046）、T分期（P = 0.031）及淋巴结浸润（P = 0.045）相关。NRP - 1高表达的肿瘤中微血管密度显著高于NRP - 1低表达的肿瘤（每高倍视野平均为13.9[标准差，9.1]对10.2[标准差，7.2]；P = 0.001）。多因素Cox回归分析表明，NRP - 1高表达与总生存期缩短独立相关（风险比，2.10；95%置信区间，1.19 - 3.70）。