Ben Qiwen, Zheng Jianming, Fei Jian, An Wei, Li Ping, Li Zhaoshen, Yuan Yaozong
From the *Department of Gastroenterology, Ruijin Hospital, Shanghai Jiaotong University; †Department of Pathology, Changhai Hospital of Second Military Medical University; ‡Department of General Surgery, Ruijin Hospital, Shanghai Jiaotong University; and §Department of Gastroenterology, Changhai Hospital of Second Military Medical University, Shanghai, China.
Pancreas. 2014 Jul;43(5):744-9. doi: 10.1097/MPA.0000000000000117.
Neuropilin 1 (NRP-1) appears to promote angiogenesis by acting as a coreceptor with vascular endothelial growth factor receptor. We correlated NRP-1 expression with microvessel density (MVD) and overall survival (OS) in human pancreatic ductal adenocarcinomas (PDACs).
Neuropilin 1 expression was graded semiquantitatively using immunohistochemistry in patients with resected PDAC. Moreover, MVD was determined with an anti-CD31 antibody staining. Expression of NRP-1 was correlated with MVD and clinicopathologic features in patients with PDAC. Overall survival effects of NRP-1 expression were evaluated by multivariate Cox regression and Kaplan-Meier analyses.
High NRP-1 expression was associated with advanced Union for International Cancer Control stage (P = 0.046), T stage (P = 0.031), and lymph node invasion (P = 0.045). Microvessel density was significantly higher in the tumors with high NRP-1 expression than that in the tumors with low NRP-1 expression (mean, 13.9 [SD, 9.1] vs 10.2 [SD, 7.2] per high-power field; P = 0.001). The multivariate Cox regression analysis demonstrated that high NRP-1 expression was independently associated with reduced OS (hazard ratio, 2.10; 95% confidence interval, 1.19-3.70).
Neuropilin 1 is highly expressed in PDACs, and high expression of NRP-1 is significantly correlated with angiogenesis, advanced tumor-node-metastasis stage, p T stage, node invasion, and poor postoperative OS.
神经纤毛蛋白1（NRP - 1）似乎通过作为血管内皮生长因子受体的共受体来促进血管生成。我们将人胰腺导管腺癌（PDAC）中NRP - 1的表达与微血管密度（MVD）及总生存期（OS）进行了相关性分析。
对接受手术切除的PDAC患者，采用免疫组织化学方法对神经纤毛蛋白1的表达进行半定量分级。此外，用抗CD31抗体染色来测定MVD。将PDAC患者中NRP - 1的表达与MVD及临床病理特征进行相关性分析。通过多因素Cox回归分析和Kaplan - Meier分析评估NRP - 1表达对总生存期的影响。
NRP - 1高表达与国际癌症控制联盟晚期（P = 0.046）、T分期（P = 0.031）及淋巴结浸润（P = 0.045）相关。NRP - 1高表达的肿瘤中微血管密度显著高于NRP - 1低表达的肿瘤（每高倍视野平均为13.9[标准差，9.1]对10.2[标准差，7.2]；P = 0.001）。多因素Cox回归分析表明，NRP - 1高表达与总生存期缩短独立相关（风险比，2.10；95%置信区间，1.19 - 3.70）。
神经纤毛蛋白1在PDAC中高表达，且NRP - 1高表达与血管生成、晚期肿瘤 - 淋巴结 - 转移分期、pT分期、淋巴结浸润及术后总生存期差显著相关。
