Mishra Mahendra Narain, Dudeja Puja, Gupta Rakesh Kumar
Dr Lal Path Labs, National Reference Laboratory, Rohini, New Delhi, India, e-mail:
Iran J Immunol. 2014 Mar;11(1):21-8.
Pediatric bronchial asthma is associated with considerable morbidity. The study was carried out to examine the association of Human Leukocyte Antigen (HLA)-Class II with the disease as we found no similar study on Asian Indian population.
To define the HLA-Class II antigens in Asian Indian pediatric patients with asthma.
A total of 103 children with asthma and 152 controls were analysed for HLA Class II (DRB1, DQB1 and DPB1) by PCR-SSP (Sequence Specific Primers) method. Total serum IgE levels were determined by ELISA assay.
A positive family history was recorded in 59 patients (57%) and 13 (8.5%) of healthy controls. Serum IgE levels were more than normal range in 72% of the patients and 33% of healthy subjects with mean values of 4877 and 627 IU/ml, respectively. DRB1 04 and DQB1 03 showed significant positive relations while DRB1 15 showed a negative association with asthma. DQB1 02 was more common in healthy individuals but was not statistically significant.
A positive association of the DR4/DQB1 03 and a negative association of DRB1 15 was seen with extrinsic bronchial asthma. However, more studies are required on larger populations to confirm the association of HLA Class II alleles in Indians before a particular allele can be labeled as being protective or causative for asthma.
儿童支气管哮喘与相当高的发病率相关。由于我们未发现关于亚洲印度人群的类似研究，因此开展了此项研究以检验人类白细胞抗原（HLA）-II类与该疾病的关联。
确定亚洲印度裔哮喘儿童患者中的HLA-II类抗原。
采用聚合酶链反应-序列特异性引物（PCR-SSP）方法，对103例哮喘儿童和152例对照进行HLA-II类（DRB1、DQB1和DPB1）分析。通过酶联免疫吸附测定（ELISA）法测定血清总IgE水平。
59例患者（57%）和13例健康对照（8.5%）有阳性家族史。72%的患者和33%的健康受试者血清IgE水平高于正常范围，平均值分别为4877和627 IU/ml。DRB1 04和DQB1 03与哮喘呈显著正相关，而DRB1 15与哮喘呈负相关。DQB1 02在健康个体中更常见，但无统计学意义。
外源性支气管哮喘与DR4/DQB1 03呈正相关，与DRB1 15呈负相关。然而，在将特定等位基因标记为对哮喘具有保护作用或致病作用之前，需要对更多人群进行研究以证实印度人群中HLA-II类等位基因的关联。
