Mubarak Bushra, Afzal Nadeem, Javaid Khursheed, Talib Rakhshanda, Aslam Rabia, Latif Waqas, Khaliq Saba
Department of Immunology, University of Health Sciences, Lahore, Pakistan.
Department of Immunology, University of Health Sciences, Lahore, Pakistan AND Department of Physiology and Cell Biology, University of Health Sciences, Lahore, Pakistan.
Iran J Allergy Asthma Immunol. 2017 Aug;16(4):313-320.
In Pakistan about 3.7% of the population is suffering from asthma, a chronic inflammatory disorder of airways. Asthma has wide spectrum of predisposing factors including environment and genetics. Many studies have been performed to determine association of asthma with serum IgE and major histocompatibility complex (MHC) alleles but conflicting results were reported. Therefore, present study was designed to determine frequency of HLA-DQβ10201 and DQβ10301 alleles in patients with bronchial asthma. This case control study included 85 asthmatic patients and 85 healthy controls. HLA-DQβ10201 and DQβ10301 alleles were detected by allele specific PCR and serum IgE was determined by ELISA. Median and inter-quartile range (IQR) of total IgE level were more increased in asthma patients (585.7 IU/mL and 247.2-848.1 IU/mL) compared to healthy controls (65.1 IU/mL and 28.1-181.3 IU/mL) (p<0.001). Frequency of HLA-DQβ10201 and -DQβ10301 alleles was more in healthy controls (32% and 38%, p=0.616) as compared to bronchial asthma patients (28% and 26%, p= 0.09). There was a significant association of IgE levels and HLA-DQβ10201 allele. Patients positive for HLA-DQβ10201 allele had low level of serum IgE 357.2 IU/mL (153.9-634.3 IU/mL) compared to the patients negative for this HLA allele i.e. 642.9 IU/mL (289.8-1299.5IU/mL) (p=0.005), whereas, HLA-DQβ10301 allele was not associated with total serum IgE level (p=0.865). Our findings show that HLA-DQβ10201 and -DQβ10301 alleles were not associated with asthma; however, HLA-DQβ10201 allele was associated with low levels of total serum IgE in the study population.
在巴基斯坦，约3.7%的人口患有哮喘，这是一种气道慢性炎症性疾病。哮喘有多种诱发因素，包括环境和遗传因素。已经进行了许多研究来确定哮喘与血清免疫球蛋白E（IgE）和主要组织相容性复合体（MHC）等位基因之间的关联，但报道的结果相互矛盾。因此，本研究旨在确定支气管哮喘患者中人类白细胞抗原-DQβ10201和DQβ10301等位基因的频率。这项病例对照研究纳入了85例哮喘患者和85名健康对照者。通过等位基因特异性聚合酶链反应（PCR）检测HLA-DQβ10201和DQβ10301等位基因，采用酶联免疫吸附测定（ELISA）法测定血清IgE。与健康对照者（65.1 IU/mL，四分位间距为28.1 - 181.3 IU/mL）相比，哮喘患者的总IgE水平中位数和四分位间距（IQR）更高（585.7 IU/mL，四分位间距为247.2 - 848.1 IU/mL）（p<0.001）。与支气管哮喘患者（28%和26%，p = 0.09）相比，健康对照者中HLA-DQβ10201和-DQβ10301等位基因的频率更高（32%和38%，p = 0.616）。IgE水平与HLA-DQβ10201等位基因之间存在显著关联。与该HLA等位基因阴性的患者（642.9 IU/mL，四分位间距为289.8 - 1299.5IU/mL）相比，HLA-DQβ10201等位基因阳性的患者血清IgE水平较低，为357.2 IU/mL（四分位间距为153.9 - 634.3 IU/mL）（p = 0.005），而HLA-DQβ10301等位基因与血清总IgE水平无关（p = 0.865）。我们的研究结果表明，HLA-DQβ10201和-DQβ10301等位基因与哮喘无关；然而，在研究人群中，HLA-DQβ10201等位基因与血清总IgE水平较低有关。
