1] Center for Nanoparticle Research, Institute for Basic Science (IBS), Seoul National University, Gwanakro, Gwanak-gu, Seoul 151-744, South Korea [2] Department of Electrical and Computer Engineering, Seoul National University, Gwanakro, Gwanak-gu, Seoul 151-744, South Korea [3] Inter-university Semiconductor Research Center, Seoul National University, Gwanakro, Gwanak-gu, Seoul 151-744, South Korea [4].
1] Center for Nanoparticle Research, Institute for Basic Science (IBS), Seoul National University, Gwanakro, Gwanak-gu, Seoul 151-744, South Korea [2] Inter-university Semiconductor Research Center, Seoul National University, Gwanakro, Gwanak-gu, Seoul 151-744, South Korea [3] Interdisciplinary program of Bioengineering, Seoul National University, Gwanakro 1, Gwanak-gu, Seoul 151-744, South Korea.
Nat Commun. 2014 Mar 17;5:3468. doi: 10.1038/ncomms4468.
One quantitative liquid handling method in conventional assay processes is pipetting, which delivers a precise volume of one sample at a time. As this process becomes laborious and time-consuming as the number of samples increases, researchers in individual laboratories need a way to conduct large-scale assays in a reasonable amount of time and at an affordable cost. Here we report a novel handling technique of chemical substances termed 'partipetting', which allows the one-step pipetting of various chemical-laden hydrogels. We pipette and assemble various types of encoded chemical-laden microparticles in microwell arrays in parallel. The combination of this heterogeneous particle chip and a cell chip induces the release of the chemicals from the hydrogels and, eventually, the chemicals treat the targets. Based on bioassay applications using partipetting, we show its capability in large-scale bioassays, without the need for high-throughput bioassay resources, owing to a reduction in the assay costs and time.
传统分析过程中的一种定量液体处理方法是移液，它可以一次精确地输送一个样本的精确体积。随着样本数量的增加，这个过程变得繁琐和耗时，因此各个实验室的研究人员需要一种方法，以便在合理的时间内以可承受的成本进行大规模分析。在这里，我们报告了一种称为“分区移液”的新型化学物质处理技术，它允许一步式移取各种负载化学物质的水凝胶。我们可以并行地在微孔阵列中分区移取和组装各种类型的编码化学物质负载的微球。这种异质粒子芯片和细胞芯片的组合可以从水凝胶中释放出化学物质，最终化学物质可以处理目标。基于使用分区移液进行的生物分析应用，我们展示了它在大规模生物分析中的能力，由于分析成本和时间的减少，而无需高通量生物分析资源。
