Muñoz Hector Enrique, Che James, Kong Janay Elise, Di Carlo Dino
Department of Bioengineering, California NanoSystems Institute, Jonsson Comprehensive Cancer Center, University of California Los Angeles, 420 Westwood Plaza, 5121 Engineering V, Box 951600, Los Angeles, California 90095, USA.
Lab Chip. 2014 Jul 7;14(13):2212-6. doi: 10.1039/c4lc90042a. Epub 2014 May 22.
Here we highlight emerging technologies in the synthesis, handling, and application of encoded microparticles for multiplexed assays. Traditionally, in drug discovery and life sciences research, multiple reactions will be conducted in parallel using microwell plate formats or microfluidic implementations, in which volumes are confined and reactions annotated by knowledge of what reagents were added to each volume. Microparticle-based information carriers provide an alternative approach to performing such multiplexed reactions, in which reactions and events are instead annotated with unique codes associated with the solid-phase particle. One challenge has been in creating a unique and large enough code set that is also easily readout, and we highlight two approaches that have brought orthogonal optical tagging techniques to bear. Another challenge has been that in such approaches, reactions have usually been confined to the surface of, or within the bulk of the specifically-tagged particle. We also highlight a creative approach and strategy for multiplexing - called "partipetting"- in which the coded particle can be a carrier of a unique fluid reagent.
在此，我们重点介绍用于多重分析的编码微颗粒在合成、处理及应用方面的新兴技术。传统上，在药物研发和生命科学研究中，多重反应会使用微孔板形式或微流控装置并行进行，其中反应体积受限，且通过知晓添加到每个体积中的试剂来标注反应。基于微颗粒的信息载体为进行此类多重反应提供了一种替代方法，在这种方法中，反应和事件是通过与固相颗粒相关的独特代码来标注的。一个挑战在于创建一个独特且足够大的代码集，同时该代码集还易于读出，我们重点介绍了两种应用正交光学标记技术的方法。另一个挑战是，在这些方法中，反应通常局限于特定标记颗粒的表面或主体内部。我们还重点介绍了一种用于多重分析的创新方法和策略——“颗粒移液法”，其中编码颗粒可以是独特流体试剂的载体。
