Hoffenbach A, Langlade-Demoyen P, Dadaglio G, Vilmer E, Michel F, Mayaud C, Autran B, Plata F
Laboratoire de Biologie et d'Immunologie Moléculaires des Rétrovirus, Institut Pasteur, Paris, France.
J Immunol. 1989 Jan 15;142(2):452-62.
CTL specific for the HIV belong to the CD8 subset of T lymphocytes, and their activity is restricted by class I HLA transplantation Ag. In this report, HIV-specific CTL and their precursor cells were quantified by limiting dilution analysis. CTL were recovered from the lungs, lymph nodes, and blood of asymptomatic seropositive carriers and of patients with AIDS. HIV was found to be very immunogenic. High frequencies of both HIV-specific CTL and CTL precursor cells were detected in infected individuals. These CTL killed autologous HIV-infected macrophages and T4 lymphoblasts. They also killed doubly transfected P815-A2-env-LAV mouse tumor cells, which express the human HLA-A2 gene and the HIV-1 env gene. In the longitudinal studies of two HIV-infected patients, CTL and CTL precursor cell frequencies decreased as the clinical and immunologic status of the patients deteriorated. Most surprisingly, PBL from seronegative donors also responded to HIV stimulation in vitro and generated large numbers of HLA-restricted, HIV-specific CTL.
针对HIV的细胞毒性T淋巴细胞（CTL）属于T淋巴细胞的CD8亚群，其活性受I类人类白细胞抗原（HLA）移植抗原的限制。在本报告中，通过有限稀释分析对HIV特异性CTL及其前体细胞进行了定量。CTL取自无症状血清阳性携带者以及艾滋病患者的肺、淋巴结和血液。发现HIV具有很强的免疫原性。在受感染个体中检测到高频率的HIV特异性CTL和CTL前体细胞。这些CTL可杀死自体HIV感染的巨噬细胞和T4淋巴母细胞。它们还能杀死双转染的P815 - A2 - env - LAV小鼠肿瘤细胞，该细胞表达人类HLA - A2基因和HIV - 1 env基因。在对两名HIV感染患者的纵向研究中，随着患者临床和免疫状态的恶化，CTL和CTL前体细胞频率降低。最令人惊讶的是，来自血清阴性供体的外周血淋巴细胞（PBL）在体外也对HIV刺激产生反应，并产生大量HLA限制的、HIV特异性CTL。
