Yu Jun-Jie, Wu Yin-Xia, Zhao Fu-Jun, Xia Shu-Jie
Department of Urology, First People's Hospital, Shanghai Jiao Tong University, Shanghai, 200080, People's Republic of China.
Med Oncol. 2014 Apr;31(4):910. doi: 10.1007/s12032-014-0910-y. Epub 2014 Mar 15.
The present study aimed to investigate the biological functions of miR-96 in the processes of proliferation and clonogenicity in the prostate cancer cells. miR-96 was identified to be markedly up-regulated in prostate cancer cell and cancer tissues compared with normal prostate cell and normal prostate tissues by microarray method and RT-PCR analysis. Down-regulation of miR-96 expression reduced the proliferation and colony formation ability of PC3 prostate cancer cells, while over-expression of miR-96 induced proliferation and colony formation ability of LNCaP prostate cancer cells. Forkhead box protein O1 (FOXO1) is key tumor suppressors and has been shown to play key roles in the regulation of diverse cellular processes, including cell proliferation, differentiation, cell cycle progression and apoptosis. The expression level of FOXO1 was strikingly up-regulated in PC3 cells after transfected with miR-96 inhibitor, and FOXO1 expression was down-regulated in LNCaP cells after transfected with miR-96 mimics. miR-96 may play a vital role in promoting cell proliferation in human prostate cancer cells. Inhibition of miR-96 caused expression increase of tumor suppressor gene FOXO1, thus manipulating miR-96 expression may be a promising approach in treatment of prostate cancer.
本研究旨在探讨miR-96在前列腺癌细胞增殖和克隆形成过程中的生物学功能。通过微阵列法和逆转录-聚合酶链反应（RT-PCR）分析发现，与正常前列腺细胞和正常前列腺组织相比，miR-96在前列腺癌细胞和癌组织中显著上调。下调miR-96表达可降低PC3前列腺癌细胞的增殖和集落形成能力，而过表达miR-96则可诱导LNCaP前列腺癌细胞的增殖和集落形成能力。叉头框蛋白O1（FOXO1）是关键的肿瘤抑制因子，已被证明在多种细胞过程的调控中发挥关键作用，包括细胞增殖、分化、细胞周期进程和凋亡。用miR-96抑制剂转染后，PC3细胞中FOXO1的表达水平显著上调，而用miR-96模拟物转染后，LNCaP细胞中FOXO1的表达下调。miR-96可能在促进人前列腺癌细胞增殖中起重要作用。抑制miR-96可导致肿瘤抑制基因FOXO1的表达增加，因此调控miR-96的表达可能是治疗前列腺癌的一种有前景的方法。
