Chang Fred, Atilgan Erdinc, Burgess David, Minc Nicolas
Department of Microbiology and Immunology, Columbia University Medical Center, 701 W. 168 Street, New York, NY, 10032, USA,
Methods Mol Biol. 2014;1136:281-90. doi: 10.1007/978-1-4939-0329-0_13.
Cell shape is an important cellular parameter that influences the spatial organization and function of cells. However, it has often been challenging to study the effects of cell shape because of difficulties in experimentally controlling cell shape in a defined way. We describe here a method of physically manipulating sea urchin cells into specified shapes by inserting them into micro-fabricated chambers of different shapes. This method allows for generation of large systematic and quantitative data sets and may be adaptable for different cell types and contexts.
细胞形状是一个重要的细胞参数，它影响细胞的空间组织和功能。然而，由于难以以明确的方式通过实验控制细胞形状，研究细胞形状的影响往往具有挑战性。我们在此描述一种通过将海胆细胞插入不同形状的微加工腔室中来将其物理操纵成特定形状的方法。这种方法能够生成大量系统的定量数据集，并且可能适用于不同的细胞类型和环境。
