Yang Y, Xu J R, Liu X M, Zhou J, Yang B, Li M, Wang Y J
Key Laboratory of the Ministry of Education for Conservation and Utilization of Special Biological Resources in Western China, Yinchuan, China.
Key Laboratory of the Ministry of Education for Conservation and Utilization of Special Biological Resources in Western China, Yinchuan, China
Genet Mol Res. 2014 Feb 27;13(1):1212-9. doi: 10.4238/2014.February.27.6.
In the present study, the correlation of polymorphisms of the apolipoprotein E (apoE) gene with the susceptibility of essential hypertension (EH) was investigated. Single nucleotide polymorphisms of the apoE gene at the -491 A>T, +969 C>G, and +2836 G>A sites were determined in 221 non-EH individuals and 109 subjects with EH of Chinese Hui ethnicity using polymerase chain reaction-restriction fragment length polymorphism analysis. The results showed that neither the genotypic frequency nor the allelic frequency at the -491 A>T and +969 C>G sites exhibited a statistically significant difference between these two groups (P > 0.05). However, a significant difference was observed in genotypic frequency and allelic frequency at the +2836 G>A site between EH patients and non-EH individuals (P < 0.01). In addition, a significantly higher frequency of the A allele at the +2836 G>A site was also detected in EH patients (83%) compared with controls (47.5%) (P < 0.01; OR = 4.82, 95%CI = 3.25-7.17); in contrast, the frequency of the G allele at the +2836 G>A site was significantly lower (17%) in the patient group in comparison with the non-EH cohorts (52.5%) (P < 0.01; OR = 0.21, 95%CI = 0.14-0.31). These results suggest that the polymorphism at the +2836 G>A site in the apoE gene is strongly correlated with the susceptibility to EH in the Chinese Hui ethnic population.
在本研究中，调查了载脂蛋白E（apoE）基因多态性与原发性高血压（EH）易感性之间的相关性。采用聚合酶链反应-限制性片段长度多态性分析，对221名非EH个体和109名中国回族EH患者的apoE基因在-491 A>T、+969 C>G和+2836 G>A位点的单核苷酸多态性进行了测定。结果显示，这两个位点的基因型频率和等位基因频率在两组之间均无统计学显著差异（P>0.05）。然而，在EH患者和非EH个体之间，+2836 G>A位点的基因型频率和等位基因频率存在显著差异（P<0.01）。此外，与对照组（47.5%）相比，在EH患者中也检测到+2836 G>A位点A等位基因的频率显著更高（83%）（P<0.01；OR=4.82，95%CI=3.25-7.17）；相反，与非EH队列（52.5%）相比，患者组中+2836 G>A位点G等位基因的频率显著更低（17%）（P<0.01；OR=0.21，95%CI=0.14-0.31）。这些结果表明，apoE基因+2836 G>A位点的多态性与中国回族人群对EH的易感性密切相关。
