Park Ji Won, Ku Sook Hee, Moon Hyung-Ho, Lee Minhyung, Choi Donghoon, Yang Jaemoon, Huh Yong-Min, Jeong Ji Hoon, Park Tae Gwan, Mok Hyejung, Kim Sun Hwa
Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, 305-701, Republic of Korea.
Macromol Biosci. 2014 Mar;14(3):380-9. doi: 10.1002/mabi.201300340. Epub 2013 Oct 8.
Poly(ethylene glycol)-coated cross-linked iron oxide nanoparticles (PCIONs) are developed for therapeutic engineering of mesenchymal stem cells (MSCs) and their monitoring via magnetic resonance (MR) imaging at a time. PCIONs successfully combine with plasmid DNA (pDNA) via ionic interaction. Accordingly, PCION/pDNA complexes mediate superior translocations of vascular endothelial growth factor (VEGF) pDNA into intracellular regions of MSCs under external magnetic field, which significantly elevate production of VEGF from MSCs. Genetically engineered MSCs are also clearly visualized via MR imaging after administration to rat cerebrovascular ischemia models, which enable tracking of MSCs migration from injected sites to injured ischemic area.
聚乙二醇包被的交联氧化铁纳米颗粒（PCIONs）被开发用于间充质干细胞（MSCs）的治疗工程以及同时通过磁共振（MR）成像对其进行监测。PCIONs通过离子相互作用成功地与质粒DNA（pDNA）结合。因此，在外部磁场作用下，PCION/pDNA复合物介导血管内皮生长因子（VEGF）pDNA向MSCs细胞内区域的高效转运，这显著提高了MSCs中VEGF的产生。在给予大鼠脑血管缺血模型后，经基因工程改造的MSCs也能通过MR成像清晰地可视化，这使得能够追踪MSCs从注射部位迁移到受损缺血区域的过程。
