Conte M, Targueta C P, Zucchi M I, Souza A P, Recco-Pimentel S M
Departamento de Biologia Estrutural e Funcional, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, SP, Brasil.
Polo Regional Centro Sul, Agência Paulista de Tecnologia dos Agronegócios, Piracicaba, SP, Brasil.
Genet Mol Res. 2014 Feb 21;13(3):8055-65. doi: 10.4238/2014.February.21.4.
The barker frog Physalaemus cuvieri is widely distributed in South America and is found in all regions of Brazil. Significant intraspecific morphological variation in this species has been reported. To determine the genetic structure of the natural Brazilian populations of P. cuvieri, 10 different populations geographically separated by 99.41 to 2936.75 km were evaluated using 10 polymorphic microsatellite loci. In addition, mitochondrial DNA data were analyzed to determine genetic distance between the populations. The genetic variation was found to be significant in most of the populations (HE ranged from 0.40 to 0.59, and allelic richness ranged from 2.07 to 3.54). An FST value of 0.27 indicated that high genetic structure was present among the P. cuvieri populations. STRUCTURE analyses grouped the 10 populations into nine clusters and indicated that only two of the populations were not genetically differentiated. The genetic distance calculated from the mitochondrial DNA data showed values <0.03 for seven of the populations.
巴克尔蛙（Physalaemus cuvieri）广泛分布于南美洲，在巴西的所有地区都有发现。该物种存在显著的种内形态变异。为了确定巴西自然种群中巴克尔蛙的遗传结构，利用10个多态性微卫星位点对地理距离在99.41至2936.75公里之间的10个不同种群进行了评估。此外，还分析了线粒体DNA数据以确定种群之间的遗传距离。在大多数种群中发现了显著的遗传变异（杂合度范围为0.40至0.59，等位基因丰富度范围为2.07至3.54）。FST值为0.27表明巴克尔蛙种群之间存在高度的遗传结构。STRUCTURE分析将10个种群分为9个簇，并表明只有两个种群没有遗传分化。根据线粒体DNA数据计算出的7个种群的遗传距离值<0.03。
