Ahola Susanna, Telkki Ville-Veikko
Department of Physics, NMR Research Group, University of Oulu, P.O.Box 3000, FIN-90014 (Finland).
Chemphyschem. 2014 Jun 6;15(8):1687-92. doi: 10.1002/cphc.201301117. Epub 2014 Mar 13.
Nuclear spin-lattice (T1) and spin-spin (T2) relaxation times provide versatile information about the dynamics and structure of substances, such as proteins, polymers, porous media, and so forth. Multidimensional experiments increase the information content and resolution of NMR relaxometry, but they also multiply the measurement time. To overcome this issue, we present an efficient strategy for a single-scan measurement of a 2D T1-T2 correlation map. The method shortens the experimental time by one to three orders of magnitude as compared to the conventional method, offering an unprecedented opportunity to study molecular processes in real-time. We demonstrate that, despite the tremendous speed-up, the T1-T2 correlation maps determined by the single-scan method are in good agreement with the maps measured by the conventional method. The concept of the single-scan T1-T2 correlation experiment is applicable to a broad range of other multidimensional relaxation and diffusion experiments.
核自旋 - 晶格（T1）和自旋 - 自旋（T2）弛豫时间提供了关于诸如蛋白质、聚合物、多孔介质等物质的动力学和结构的丰富信息。多维实验增加了核磁共振弛豫测量的信息含量和分辨率，但同时也成倍增加了测量时间。为克服这一问题，我们提出了一种用于二维T1 - T2相关图谱单扫描测量的高效策略。与传统方法相比，该方法将实验时间缩短了一到三个数量级，为实时研究分子过程提供了前所未有的机会。我们证明，尽管速度大幅提升，但通过单扫描方法确定的T1 - T2相关图谱与传统方法测量的图谱高度吻合。单扫描T1 - T2相关实验的概念适用于广泛的其他多维弛豫和扩散实验。
