Dong Sheying, Huang Guiqi, Lu Jinsuo, Huang Tinglin
College of Sciences, Xi-an University of Architecture and Technology, Xi-an, China; School of Environmental and Municipal Engineering, Xi-an University of Architecture and Technology, Xi'an, China.
J Sep Sci. 2014 Jun;37(11):1337-42. doi: 10.1002/jssc.201400010. Epub 2014 Apr 13.
A dispersive liquid-liquid microextraction procedure based on solidification of floating organic droplet has been investigated for the determination of fungicides (cyprodinil, difenoconazole, myclobutanil, and spiroxamine) in sediments by HPLC with diode array detection. In the overall extraction process, the extraction solvents can be separated easily from the sample solution, and the experiment time was shortened. Moreover, several parameters such as the type and volume of the extraction solvent and dispersive solvent, centrifugal speed, extraction time, and salt effect that affect the extraction efficiencies of the target fungicides were studied and optimized. Under the optimized conditions, the LOD for the target analytes were in the range of 0.1-0.5 μg/g. Satisfactory recoveries of the target analytes in the sediment samples were 81.00-99.00%, with RSDs (n = 5) that ranged from 1.8 to 6.5%. Finally, the simple, sensitive, and environmentally friendly method was successfully applied to determine the target fungicides in actual sediment samples.
本文研究了一种基于浮滴固化的分散液液微萃取方法，用于通过高效液相色谱-二极管阵列检测法测定沉积物中的杀菌剂（嘧菌环胺、苯醚甲环唑、腈菌唑和螺螨酯）。在整个萃取过程中，萃取溶剂能很容易地与样品溶液分离，缩短了实验时间。此外，研究并优化了影响目标杀菌剂萃取效率的几个参数，如萃取溶剂和分散溶剂的类型和体积、离心速度、萃取时间和盐效应。在优化条件下，目标分析物的检出限为0.1 - 0.5 μg/g。沉积物样品中目标分析物的回收率令人满意，为81.00 - 99.00%，相对标准偏差（n = 5）为1.8%至6.5%。最后，该简单、灵敏且环保的方法成功应用于实际沉积物样品中目标杀菌剂的测定。
