Fornace A J, Schalch H, Alamo I
Radiation Oncology Branch, National Cancer Institute, Bethesda, Maryland 20892.
Mol Cell Biol. 1988 Nov;8(11):4716-20. doi: 10.1128/mcb.8.11.4716-4720.1988.
Sequence analysis of Chinese hamster V79 lung fibroblast cDNA clones, which code for UV radiation-inducible transcripts, revealed that many of the clones corresponded to metallothioneins (MTs) I and II. A third cDNA clone, DDIU4, was found also to code for a similar-size UV-inducible transcript which was unrelated to MT by both sequence analysis and kinetics of induction. MTI and MTII RNAs rapidly increased in V79 cells within 1 h after UV irradiation, and maximum induction was seen by 4 h. This rapid induction of MT RNA by UV irradiation was not observed in human fibroblasts. MTI and MTII were coordinately induced in both time course and dose-response experiments, although the induction of MTII, up to 30-fold, was three to four times greater than that of MTI. The induction of MT did not appear to be a general stress response, since no increase occurred after exposure to X rays or H2O2.
对编码紫外线诱导转录本的中国仓鼠V79肺成纤维细胞cDNA克隆进行序列分析，结果显示许多克隆对应于金属硫蛋白（MTs）I和II。还发现第三个cDNA克隆DDIU4也编码一个大小相似的紫外线诱导转录本，通过序列分析和诱导动力学研究，该转录本与MT无关。MTI和MTII RNA在紫外线照射后1小时内V79细胞中迅速增加，4小时时达到最大诱导水平。在人成纤维细胞中未观察到紫外线照射对MT RNA的这种快速诱导。在时间进程和剂量反应实验中，MTI和MTII均被协同诱导，尽管MTII的诱导倍数高达30倍，是MTI的三到四倍。MT的诱导似乎不是一种普遍的应激反应，因为暴露于X射线或H2O2后未出现增加。
