Department of Pathology, Deen Dayal Upadhyay Hospital, Hari Nagar, New Delhi 110066, India.
Department of Pathology, Maulana Azad Medical College, Bahadur Shah Zafar Marg, New Delhi 110002, India.
J Oncol. 2014;2014:702527. doi: 10.1155/2014/702527. Epub 2014 Jan 30.
Background. Study of tumor molecular characteristics is necessary to understand both the risk of breast cancer recurrence and the response to therapy. Aims. To evaluate p53, HoxD10, and E-cadherin status in breast cancer and to correlate with histological grade and other prognostic factors. Material and Methods. The study was conducted in 60 cases of invasive ductal carcinoma NOS with 20 cases belonging to each grade and evaluation of p53 was done by IHC and that of HoxD10 and E Cadherin status by PCR and correlation was done with histological grade and other prognostic factors. Result. p53 expression was seen in 71.67% (43/60) of the tumors. HoxD10 gene was downregulated in 46.67% (28/60) of the tumors. p53 overexpression and lower HoxD10 mRNA levels showed statistically significant association higher histological grade of the tumor (P < 0.05). CDH1 gene mutation was seen in 60% (15/25) of the tumors. No significant association was found between p53 expression, HoxD10 gene, CDH1 gene mutation, and other prognostic factors. Conclusion. p53 over expression and lower HoxD10 mRNA levels were found to be significantly associated with higher grade tumours. This suggests that p53 and HoxD10 gene play an important tumor suppressor role and the loss of which results in breast cancer progression.
研究肿瘤分子特征对于了解乳腺癌复发的风险和对治疗的反应是必要的。
评估乳腺癌中 p53、HoxD10 和 E-钙黏蛋白的状态，并与组织学分级和其他预后因素相关联。
本研究在 60 例浸润性导管癌 NOS 病例中进行，其中 20 例属于每个分级，并通过免疫组织化学评估 p53 的状态，通过 PCR 评估 HoxD10 和 E 钙黏蛋白的状态，并与组织学分级和其他预后因素相关联。
p53 的表达在 71.67%（43/60）的肿瘤中可见。HoxD10 基因在 46.67%（28/60）的肿瘤中下调。p53 过表达和较低的 HoxD10 mRNA 水平与肿瘤的较高组织学分级具有统计学显著相关性（P<0.05）。在 60%（15/25）的肿瘤中可见 CDH1 基因突变。p53 表达、HoxD10 基因、CDH1 基因突变与其他预后因素之间未发现显著相关性。
p53 过表达和较低的 HoxD10 mRNA 水平与较高分级的肿瘤显著相关。这表明 p53 和 HoxD10 基因在肿瘤抑制中起着重要作用，它们的缺失导致乳腺癌的进展。
