Inoue Keisuke, Aomatsu Tomoki, Yoden Atsushi, Okuhira Takeru, Kaji Emiri, Tamai Hiroshi
Department of Pediatrics, Osaka Medical College, Takatsuki, Osaka, Japan.
J Gastroenterol Hepatol. 2014;29(7):1406-12. doi: 10.1111/jgh.12578.
Fecal calprotectin (FC) has become a reliable biomarker for intestinal inflammation in inflammatory bowel diseases (IBDs). However, a simple and rapid assay to replace conventional ELISA is necessary for wider use in clinical practice. In this study, we investigated the usefulness of a novel method for measuring FC using a colloidal gold aggregation (CGA) assay for assessing mucosal inflammation in pediatric IBDs.
FC levels were determined by ELISA and CGA assay in 309 fecal samples (ulcerative colitis [UC]: 131; Crohn's disease [CD]: 121; healthy controls: 57). For endoscopic evaluation, the modified Matts' grading system for UC and the simple endoscopic score for CD were used.
A strong correlation was found between the FC values determined by the two methods (r = 0.98, P < 0.01). FC levels, determined by CGA assay, strongly correlated with the endoscopic score for UC (r = 0.70, P < 0.01) and CD (r = 0.58, P < 0.01). In the UC patients with endoscopic remission, the FC levels determined by CGA assay (median: 31.5 μg/g, n = 14) were as low as in healthy controls. For patients in clinical remission but showing an active status endoscopically, FC was more likely to be abnormal than commonly used laboratory markers.
Our simple and rapid assay system has excellent performance for assessing mucosal inflammation of IBDs and can be replaced for ELISA. Practical application of this assay system enables us to use FC measurement more widely in clinical practice.
粪便钙卫蛋白（FC）已成为炎症性肠病（IBD）肠道炎症的可靠生物标志物。然而，需要一种简单快速的检测方法来替代传统酶联免疫吸附测定（ELISA），以便在临床实践中更广泛地应用。在本研究中，我们调查了一种使用胶体金聚集（CGA）测定法测量FC以评估儿童IBD黏膜炎症的新方法的实用性。
采用ELISA和CGA测定法测定309份粪便样本中的FC水平（溃疡性结肠炎[UC]：131例；克罗恩病[CD]：121例；健康对照：57例）。内镜评估采用UC改良的马茨分级系统和CD简单内镜评分。
两种方法测定的FC值之间存在强相关性（r = 0.98，P < 0.01）。通过CGA测定法测定的FC水平与UC（r = 0.70，P < 0.01）和CD（r = 0.58，P < 0.01）的内镜评分密切相关。在内镜缓解的UC患者中，通过CGA测定法测定的FC水平（中位数：31.5μg/g，n = 14）与健康对照一样低。对于临床缓解但内镜显示活跃状态的患者，FC比常用实验室指标更可能异常。
我们简单快速的检测系统在评估IBD黏膜炎症方面具有优异性能，可替代ELISA。该检测系统的实际应用使我们能够在临床实践中更广泛地使用FC测量。
