Casado Javier G, Blazquez Rebeca, Jorge Inmaculada, Alvarez Veronica, Gomez-Mauricio Guadalupe, Ortega-Muñoz Mariano, Vazquez Jesus, Sanchez-Margallo Francisco M
Stem Cell Therapy Unit, Minimally Invasive Surgery Centre Jesus Uson, Caceres, Spain.
Wound Repair Regen. 2014 Mar-Apr;22(2):256-64. doi: 10.1111/wrr.12153.
Sutures are commonly used for surgical procedures and new sutures are being developed to improve wound healing. In the past decade, it has been extensively shown that mesenchymal stem cells (MSCs) have a wound healing potential. To benefit the overall wound healing process, we aimed to analyze the usage of pretreated sutures for improving the implantation of MSCs in the tissues. Our results firstly showed that suture pretreatments with gelatin, poly-L-lysine, and NaOH improved the adhesive strength of MSCs to sutures. These cells remained surrounding the sutured tissue and no significant phenotypic changes were found in those cells cultured onto pretreated sutures. In vivo experiments showed that the implantation of MSCs by suturing increases the collagen content in the sutured tissue. Moreover, proteomics analysis of secreted proteins showed that collagen alpha-1(I) chain was the most abundant collagen found. To our knowledge, this is the first report that aimed to improve the implantation of MSCs in tissue by suture pretreatments. Moreover, in vivo experiments suggest that MSC-coated sutures may enhance wound healing and tissue remodeling through the release of different collagen types being applicable for those patients that tend to have difficulty healing.
缝线常用于外科手术，并且新型缝线也在不断研发以促进伤口愈合。在过去十年中，大量研究表明间充质干细胞（MSC）具有促进伤口愈合的潜力。为了使整个伤口愈合过程受益，我们旨在分析预处理缝线在改善MSC在组织中植入方面的应用。我们的结果首先表明，用明胶、聚-L-赖氨酸和氢氧化钠对缝线进行预处理可提高MSC与缝线的黏附强度。这些细胞仍围绕在缝合组织周围，并且在培养于预处理缝线上的细胞中未发现明显的表型变化。体内实验表明，通过缝合植入MSC可增加缝合组织中的胶原蛋白含量。此外，对分泌蛋白的蛋白质组学分析表明，胶原蛋白α-1（I）链是所发现的最丰富的胶原蛋白。据我们所知，这是第一份旨在通过缝线预处理改善MSC在组织中植入的报告。此外，体内实验表明，对于那些愈合困难的患者，包被有MSC的缝线可能通过释放不同类型的胶原蛋白来促进伤口愈合和组织重塑。
