Chicharro Deborah, Carrillo Jose M, Rubio Mónica, Cugat Ramón, Cuervo Belén, Guil Silvia, Forteza Jerónimo, Moreno Victoria, Vilar Jose M, Sopena Joaquín
Bioregenerative Medicine and Applied Surgery Research Group, Animal Medicine and Surgery Department, Veterinary Faculty, Universidad Cardenal Herrera-CEU, CEU Universities, Valencia, Spain.
Orthopaedic service in Hospital Quirón, Artroscopia GC, Barcelona, Spain.
BMC Vet Res. 2018 Sep 21;14(1):288. doi: 10.1186/s12917-018-1577-y.
The use of Plasma Rich in Growth Factors (PRGF) and Adipose Derived Mesenchymal Stem Cells (ASCs) are today extensively studied in the field of regenerative medicine. In recent years, human and veterinary medicine prefer to avoid using traumatic techniques and choose low or non-invasive procedures. The objective of this study was to evaluate the efficacy of PRGF, ASCs and the combination of both in wound healing of full-thickness skin defects in rabbits. With this purpose, a total of 144 rabbits were used for this study. The animals were divided in three study groups of 48 rabbits each depending on the administered treatment: PRGF, ASCs, and PGRF+ASCs. Two wounds of 8 mm of diameter and separated from each other by 20 mm were created on the back of each rabbit: the first was treated with saline solution, and the second with the treatment assigned for each group. Macroscopic and microscopic evolution of wounds was assessed at 1, 2, 3, 5, 7 and 10 days post-surgery. With this aim, 8 animals from each treatment group and at each study time were euthanized to collect wounds for histopathological study.
Wounds treated with PRGF, ASCs and PRGF+ASCs showed significant higher wound healing and epithelialization rates, more natural aesthetic appearance, significant lower inflammatory response, significant higher collagen deposition and angiogenesis compared with control wounds. The combined treatment PRGF+ASCs showed a significant faster cutaneous wound healing process.
The combined treatment PRGF+ASCs showed the best results, suggesting this is the best choice to enhance wound healing and improve aesthetic results in acute wounds.
富含生长因子的血浆（PRGF）和脂肪来源间充质干细胞（ASCs）如今在再生医学领域得到广泛研究。近年来，人类和兽医学更倾向于避免使用创伤性技术，而选择低侵入性或非侵入性程序。本研究的目的是评估PRGF、ASCs以及两者联合使用对兔全层皮肤缺损伤口愈合的疗效。为此，本研究共使用了144只兔子。根据所给予的治疗方法，将动物分为三个研究组，每组48只兔子：PRGF组、ASCs组和PRGF + ASCs组。在每只兔子的背部制作两个直径为8毫米、彼此相距20毫米的伤口：第一个伤口用生理盐水处理，第二个伤口用为每组指定的治疗方法处理。在术后1、2、3、5、7和10天评估伤口的宏观和微观演变。为此，在每个治疗组和每个研究时间点处死8只动物，以收集伤口进行组织病理学研究。
与对照伤口相比，用PRGF、ASCs和PRGF + ASCs处理的伤口显示出显著更高的伤口愈合和上皮化率、更自然的美观外观、显著更低的炎症反应、显著更高的胶原蛋白沉积和血管生成。联合治疗PRGF + ASCs显示出明显更快的皮肤伤口愈合过程。
联合治疗PRGF + ASCs显示出最佳效果，表明这是促进急性伤口愈合和改善美观效果的最佳选择。
