Minoprio P, Burlen O, Pereira P, Guilbert B, Andrade L, Hontebeyrie-Joskowicz M, Coutinho A
Department of Immunology, Pasteur Institute, Paris, France.
Scand J Immunol. 1988 Nov;28(5):553-61. doi: 10.1111/j.1365-3083.1988.tb01487.x.
The specificity of B lymphocytes activated in the acute phase of murine Trypanosoma cruzi infection was analysed in a panel of immunoglobulin-secreting hybridomas derived by fusion of lymph node cells 7 days after intraperitoneal parasite inoculation. The immunoglobulin isotype distribution of the hybrids reflected the total plaque-forming cell (PFC) response in the animal at this point, with a predominance of IgG2a, IgM, and IgG2b. Screening of the hybridoma antibodies on parasite antigens by three independent methods (western blot, ELISA, and immunofluorescence) revealed only one (out of a total of 51) that bound a parasite molecule with an apparent molecular mass of 180 kDa. In contrast, antibodies of both IgM and IgG classes were found to react with a panel of autologous antigens. These results establish that most B cells activated by T. cruzi infection are not specific for parasite antigens and therefore indicate the relevance of analysing the totality of host responses to infection.
在腹腔接种寄生虫7天后，通过融合淋巴结细胞获得了一组分泌免疫球蛋白的杂交瘤，分析了小鼠克氏锥虫感染急性期激活的B淋巴细胞的特异性。此时，杂交瘤的免疫球蛋白同种型分布反映了动物体内总的空斑形成细胞（PFC）反应，其中IgG2a、IgM和IgG2b占优势。通过三种独立方法（蛋白质印迹法、酶联免疫吸附测定法和免疫荧光法）在寄生虫抗原上筛选杂交瘤抗体，结果显示，在总共51个杂交瘤中，只有一个与表观分子量为180 kDa的寄生虫分子结合。相反，发现IgM和IgG类抗体均能与一组自体抗原发生反应。这些结果表明，大多数由克氏锥虫感染激活的B细胞并非针对寄生虫抗原具有特异性，因此表明分析宿主对感染的整体反应具有重要意义。
