Hogenes Marieke, Huibers Manon, Kroone Chantal, de Weger Roel
Department of Pathology, University Medical Centre Utrecht, PO box 85500, 3508 GA Utrecht, The Netherlands.
Department of Pathology, University Medical Centre Utrecht, PO box 85500, 3508 GA Utrecht, The Netherlands.
Transplant Rev (Orlando). 2014 Jul;28(3):103-10. doi: 10.1016/j.trre.2014.02.002. Epub 2014 Feb 14.
The interest in the use of humanized mouse models for research topics like Graft versus Host Disease (GvHD), allograft studies and other studies to the human immune system is growing. The design of these models is still improving and enables even more complicated studies to these topics. For researchers it can be difficult to choose the best option from the current pool of available models. The decision will depend on which hypothesis needs to be tested, in which field of interest, and therefore 'the best model' will differ from one to another. In this review, we provide a guide to the most common available humanized mouse models, with regards to different mouse strains, transplantation material, transplantation techniques, pre- and post-conditioning and references to advantages and disadvantages. Also, an evaluation of experiences with humanized mouse models in studies on GvHD and allograft rejection is provided.
对于移植物抗宿主病（GvHD）、同种异体移植研究以及其他针对人类免疫系统的研究等课题，使用人源化小鼠模型进行研究的兴趣正在不断增长。这些模型的设计仍在不断改进，使得针对这些课题能够开展更加复杂的研究。对于研究人员来说，从当前可用的模型库中选择最佳选项可能会很困难。这一决定将取决于需要检验哪种假设、关注哪个领域，因此“最佳模型”会因情况而异。在本综述中，我们针对不同的小鼠品系、移植材料、移植技术、预处理和后处理，以及优缺点参考，提供了一份关于最常见的可用人源化小鼠模型的指南。此外，还提供了在GvHD和同种异体移植排斥研究中使用人源化小鼠模型的经验评估。
