Gwatkin R B, Conover J C, Collins R L, Quigley M M
Research Institute, Cleveland Clinic Foundation, OH 44195-5240.
Am J Obstet Gynecol. 1989 Jan;160(1):31-5. doi: 10.1016/0002-9378(89)90081-1.
The degree and normality of nuclear maturation were assessed with the fluorochrome Hoechst 33342 in two groups of inseminated human oocytes that had failed to undergo fertilization. Group 1 consisted of 67 oocytes from 27 patients, each of whom had at least two other oocytes that had been fertilized and had cleaved. Group 2 consisted of 65 oocytes from 14 patients, none of whose oocytes had been fertilized. In group 1, 52.3% of the oocytes were found to be immature (germinal vesicle stage or metaphase-telophase I), whereas in group 2 only 26% were found to be immature. Thus oocyte nuclear immaturity was the major cause of fertilization failure when companion oocytes were fertilized. When no oocytes of a patient were fertilized, most oocytes were found to be mature, so other factors, such as sperm dysfunction or zona binding abnormalities, must account for most of the fertilization failure in this group of patients.
在两组未受精的人类受精卵母细胞中，使用荧光染料Hoechst 33342评估核成熟的程度和正常性。第一组由来自27名患者的67个卵母细胞组成，每名患者至少有另外两个已受精并分裂的卵母细胞。第二组由来自14名患者的65个卵母细胞组成，这些患者的卵母细胞均未受精。在第一组中，发现52.3%的卵母细胞未成熟（生发泡期或中期-末期I），而在第二组中，仅26%的卵母细胞未成熟。因此，当配对的卵母细胞受精时，卵母细胞核未成熟是受精失败的主要原因。当患者的卵母细胞均未受精时，大多数卵母细胞被发现是成熟的，因此其他因素，如精子功能障碍或透明带结合异常，必定是导致这组患者受精失败的主要原因。
