Arkhipenko V I, Kuvik A P, Morozova S B, Pogorelova L Ia, Spitsa A I
Arkh Anat Gistol Embriol. 1988 Aug;95(8):57-61.
As demonstrate experiments performed on cells of the primary culture of the newborn rat kidney, injection of thyroxin stimulates, and addition of prednisolone inhibits the proliferative activity of the cells in the culture. At a combined and simultaneous administration of these two hormones, as well as at injection of thyroxin 2 h before prednisolone, the thyroxin program, stimulating the cell proliferation is expressed. When thyroxin is administered 2 h after prednisolone, during first 12 h the prednisolone program is expressed, and then the proliferative activity of the culture returns to the initial level. At the cooperative action of oppositely directed factors for realization of the cell proliferative program, an essential role play the time factor and lag-period duration for each of the hormones interacting. Exogenic RNAs, obtained from the kidneys of the rats, to whom thyroxin or prednisolone have been injected, when they (RNAs) are injected into the culture, they produce effects, similar to those that are noted at injecting these hormones into the incubation medium. Thus, injection of the hormones to rats, results in formation, by the cells of the organ, induced RNAs, capable to transfer a hormonal signal and produce a hormone-like effect at regulation of the proliferative activity of the cell culture in the obtained exogenic RNA from the organ-donor.
正如对新生大鼠肾脏原代培养细胞进行的实验所示，注射甲状腺素可刺激培养细胞的增殖活性，而添加泼尼松龙则会抑制其增殖活性。当同时联合使用这两种激素时，以及在注射泼尼松龙前2小时注射甲状腺素时，刺激细胞增殖的甲状腺素程序会得以体现。当在注射泼尼松龙后2小时注射甲状腺素时，在最初的12小时内泼尼松龙程序会得以体现，然后培养物的增殖活性会恢复到初始水平。在实现细胞增殖程序的相反作用因子的协同作用下，时间因素以及每种相互作用激素的滞后时间起着至关重要的作用。从注射过甲状腺素或泼尼松龙的大鼠肾脏中获得的外源性RNA，当将其注入培养物中时，会产生与将这些激素注入孵育培养基中时所观察到的类似效果。因此，向大鼠注射激素会导致器官细胞形成诱导性RNA，这些RNA能够传递激素信号，并在从器官供体获得的外源性RNA中调节细胞培养物的增殖活性时产生类激素效应。
