Teng C T, Pentecost B T, Chen Y H, Newbold R R, Eddy E M, McLachlan J A
Laboratory of Reproductive and Developmental Toxicology, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709.
Endocrinology. 1989 Feb;124(2):992-9. doi: 10.1210/endo-124-2-992.
The mouse mammary gland and uterus expressed the gene for the secretory protein lactotransferrin under various physiological conditions. Lactotransferrin, however, was induced by estrogen in a time- and dose-dependent fashion in the uterus of the immature mouse, but was not affected by estrogen in the mammary gland. Differences were also found in the expression of lactotransferrin in mammary glands and uteri of adult females during lactation. A high level of the protein was detected by immunocytochemistry in uterine epithelial cells 1 day after parturition, but immunoreactivity disappeared quickly thereafter. Lactotransferrin message was, however, relatively abundant in the mammary gland at the end of the lactation period. The presence of lactotransferrin in various tissues also was investigated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blot analysis. Two forms of immunoreactive material was detected by this method; a 70K band was found in uterine luminal fluid from the estrogen-stimulated immature mouse and in homogenates of lung, vagina, mammary gland, oviduct, spleen, lymph node, and uterus of the adult female mouse, and a 65K band was detected in submaxillary gland, kidney, ovary, and all of the above tissues. Brain and duodenum had no detectable immunoreactive material. A transient appearance of lactotransferrin was observed in the uterine luminal fluid of pseudopregnant mice. These changes in the level of lactotransferrin in the uterus and mammary gland under various physiological conditions suggest that the regulation of this protein's expression is tissue specific.
在各种生理条件下，小鼠乳腺和子宫均表达分泌蛋白乳铁传递蛋白的基因。然而，在未成熟小鼠的子宫中，乳铁传递蛋白可被雌激素以时间和剂量依赖性方式诱导，而在乳腺中不受雌激素影响。在成年雌性小鼠哺乳期的乳腺和子宫中，乳铁传递蛋白的表达也存在差异。产后1天，通过免疫细胞化学在子宫上皮细胞中检测到高水平的该蛋白，但此后免疫反应性迅速消失。然而，在哺乳期结束时，乳铁传递蛋白的信息在乳腺中相对丰富。还通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和蛋白质印迹分析研究了各种组织中乳铁传递蛋白的存在情况。通过该方法检测到两种形式的免疫反应性物质；在雌激素刺激的未成熟小鼠的子宫腔液以及成年雌性小鼠的肺、阴道、乳腺、输卵管、脾脏、淋巴结和子宫的匀浆中发现了一条70K的条带，在颌下腺、肾脏、卵巢以及上述所有组织中检测到一条65K的条带。脑和十二指肠未检测到免疫反应性物质。在假孕小鼠的子宫腔液中观察到乳铁传递蛋白的短暂出现。在各种生理条件下，子宫和乳腺中乳铁传递蛋白水平的这些变化表明该蛋白表达的调节具有组织特异性。
