Brancher João Armando, Pecharki Giovana Daniela, Doetzer Andrea Duarte, Medeiros Kamilla Gabriella Dos Santos, Cordeiro Júnior Carlos Alberto, Sotomaior Vanessa Santos, Bauer Peter, Trevilatto Paula Cristina
Center for Health and Biological Sciences, Pontifical Catholic University of Paraná (PUCPR), Imaculada Conceição Street 1155, 80215-901 Curitiba, PR, Brazil.
Int J Dent. 2011;2011:571726. doi: 10.1155/2011/571726. Epub 2011 Dec 8.
Regarding host aspects, there has been strong evidence for a genetic component in the etiology of caries. The salivary protein lactotransferrin (LTF) exhibits antibacterial activity, but there is no study investigating the association of polymorphisms in the promoter region of LTF gene with caries. The objective of this study was firstly to search the promoter region of the human LTF gene for variations and, if existent, to investigate the association of the identified polymorphisms with dental caries in 12-year-old students. From 687 unrelated, 12-year-old, both sex students, 50 individuals were selected and divided into two groups of extreme phenotypes according to caries experience: 25 students without (DMFT = 0) and 25 with caries experience (DMFT ≥ 4). The selection of individuals with extreme phenotypes augments the chances to find gene variations which could be associated with such phenotypes. LTF gene-putative promoter region (+39 to -1143) of the selected 50 individuals was analyzed by high-resolution melting technique. Fifteen students, 8 without (DMFT = 0) and 7 with caries experience (mean DMFT = 6.28), presented deviations of the pattern curve suggestive of gene variations and were sequenced. However, no polymorphisms were identified in the putative promoter region of the LTF gene.
关于宿主因素，有强有力的证据表明龋齿病因中存在遗传成分。唾液蛋白乳铁传递蛋白（LTF）具有抗菌活性，但尚无研究调查LTF基因启动子区域多态性与龋齿的关联。本研究的目的首先是在人类LTF基因的启动子区域寻找变异，若存在变异，则调查所鉴定的多态性与12岁学生龋齿的关联。从687名无亲缘关系的12岁男女学生中，根据龋齿经历选择50名个体并分为两组极端表型：25名无龋齿经历的学生（DMFT = 0）和25名有龋齿经历的学生（DMFT≥4）。选择极端表型个体增加了发现可能与此类表型相关的基因变异的机会。通过高分辨率熔解技术分析所选50名个体的LTF基因推定启动子区域（+39至 -1143）。15名学生，8名无龋齿经历（DMFT = 0）和7名有龋齿经历（平均DMFT = 6.28），呈现出提示基因变异的模式曲线偏差并进行了测序。然而，在LTF基因的推定启动子区域未鉴定到多态性。
