Yuan Qiao, Zhuo Chen, Yonggui Ma, Fuer Lu, Suhua Chen, Guangying Huang
Department of Integrated Traditional Chinese and Western Medicine, Tongji Hospital. Tongji Medical College, Huazhong University of Science and Technology, Wuhan, P.R. China. ; Department of Obstetrics and Gynecology, Zhongnan Hosptial of Wuhan University , Wuhan, P.R. China.
Department of Integrated Traditional Chinese and Western Medicine, Tongji Hospital. Tongji Medical College, Huazhong University of Science and Technology, Wuhan, P.R. China. ; Institute of Integrated Traditional Chinese and Western Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, P.R. China.
Iran J Reprod Med. 2013 Jul;11(7):565-76.
The most frequently used spermicide Nonoxynol-9 (N-9) in the clinic alters the vaginal flora, which will result in an increased risk of opportunistic infection. So development of a novel spermicidal and microbicidal drug appears to be inevitable. Vaginal local immune is an important part of vaginal flora. Secretory leukocyte protease inhibitor (SLPI), surfactant proteins D (SP-D), and lactoferrin (LF) are anti-microbial molecules with important roles in immune system of female vaginas.
To observe effect of a vaginal spermicide nonoxynol-9 (N-9) berberine plural gel on the expression of SLPI SP-D and LF in mice's vaginas. Materials and Methods : Female BABL/C mice were randomly divided into following 5 groups: normal control group, blank gel group, berberine gel group, 12% N-9 gel group and N-9 berberine plural gel group. Estradiol benzoate at physiological dose was done by hypodermic injection to every group's mice. After 72h, drug gels were separately injected into the mice's vaginas, while immunohistochemistry and Western blot were taken to detect the expression of the 3 indexes in mice's vaginas respectively after 24h and 72h of gel injection.
The differences in the three indexes between normal control group and blank gel group were not significant statistically (p>0.05). The expression of the three indexes in 12% N-9 gel group was decreased compared to that in blank gel group (p<0.05). The differences in the three indexes between N-9 berberine plural gel group and blank gel group were not significant statistically (p>0.05). Also, the three index's level of 24h and 72h in sub observation groups after treatment were without statistical significance (p>0.05).
Application of N-9 berberine plural gel had little impact on antimicrobial peptides in normal mice's vaginas.
临床最常用的杀精剂壬苯醇醚-9（N-9）会改变阴道菌群，导致机会性感染风险增加。因此，开发新型杀精和杀菌药物似乎势在必行。阴道局部免疫是阴道菌群的重要组成部分。分泌型白细胞蛋白酶抑制剂（SLPI）、表面活性蛋白D（SP-D）和乳铁蛋白（LF）是在女性阴道免疫系统中起重要作用的抗菌分子。
观察阴道用杀精剂壬苯醇醚-9（N-9）黄连素复合凝胶对小鼠阴道中SLPI、SP-D和LF表达的影响。材料与方法：将雌性BABL/C小鼠随机分为5组：正常对照组、空白凝胶组、黄连素凝胶组、12% N-9凝胶组和N-9黄连素复合凝胶组。对每组小鼠皮下注射生理剂量的苯甲酸雌二醇。72小时后，将药物凝胶分别注入小鼠阴道，在注入凝胶24小时和72小时后，分别采用免疫组化和蛋白质印迹法检测小鼠阴道中这3项指标的表达。
正常对照组与空白凝胶组这三项指标的差异无统计学意义（p>0.05）。12% N-9凝胶组这三项指标的表达低于空白凝胶组（p<0.05）。N-9黄连素复合凝胶组与空白凝胶组这三项指标的差异无统计学意义（p>0.05）。此外，各观察亚组治疗后24小时和72小时这三项指标水平差异无统计学意义（p>0.05）。
N-9黄连素复合凝胶的应用对正常小鼠阴道中的抗菌肽影响较小。
