Latha P, Prakasam V, Jonathan E I, Samiyappan R, Natarajan C
J Environ Biol. 2013 Jul;34(4):683-7.
Physic nut (Jatropha curcas) is an important commercial bio-diesel plant species and is being advocated for development of waste and dry land. The collar and root rot caused by Lasiodiplodia theobromae is an important soil borne disease which causes considerable yield loss in this crop. In this study, the effects of culture media, temperature, photoperiod, carbon and nitrogen sources and pH on mycelial growth and pycnidial production were evaluated. Among the growth media tested, potato dextrose agar supported the highest growth followed by potato sucrose agar and corn meal agar. Among several carbon sources tested, carboxy methyl cellulose and sucrose were found superior for growth and pycnidial production. The nitrogen sources viz., ammonium oxalate and ammonium dihydrogen phosphate were recorded maximum mycelial growth and pycnidial production. The fungus grows at pH 5.0-9.0 and optimum growth was observed at pH 7.0.
麻疯树（Jatropha curcas）是一种重要的商业生物柴油植物物种，正被倡导用于荒地和旱地的开发。由可可毛色二孢（Lasiodiplodia theobromae）引起的根颈腐烂病是一种重要的土传病害，会导致该作物产量大幅损失。在本研究中，评估了培养基、温度、光周期、碳源和氮源以及pH值对菌丝生长和分生孢子器产生的影响。在所测试的生长培养基中，马铃薯葡萄糖琼脂支持的生长最高，其次是马铃薯蔗糖琼脂和玉米粉琼脂。在测试的几种碳源中，羧甲基纤维素和蔗糖被发现对生长和分生孢子器产生更有利。氮源即草酸铵和磷酸二氢铵的菌丝生长和分生孢子器产生量最高。该真菌在pH 5.0 - 9.0下生长，在pH 7.0时观察到最佳生长。
