Saha A, Mandal P, Dasgupta S, Saha D
Mycology and Plant Pathology Laboratory, Department of Botany, University of North Bengal, Siliguri-734 013, India.
J Environ Biol. 2008 May;29(3):407-10.
Lasiodiplodia theobromae, a common tea (Camellia sinensis) pathogen, usually does not sporulate or sporulates poorly in common media, which makes spore production difficult. In this study the effects of culture media, carbon source, nitrogen source, temperature, pH and light on mycelial growth and sporulation were evaluated. Among several carbon sources tested, glucose and sucrose were found superior for growth. Potassium nitrate supplemented media showed maximum growth amongst the tested inorganic nitrogen sources while peptone produced maximum growth among the tested organic nitrogen sources. Tea root extract supplemented potato dextrose agar medium was found to be the most suitable for mycelial growth and sporulation of L. theobromae. The fungus grow at temperatures ranging from 40 to 36 degrees C, with optimum growth at 28 degrees C and no growth was noted at 40 degrees C. There was no significant effect of different light period on growth of L. theobromae, but light enhanced sporulation. The fungus grow at pH 3.0-8.0 and optimum growth was observed at pH 6.0. Tea root extract supplemented potato dextrose agar medium with pH 6.0 was the most suitable for production of conidia of L. theobromae at 28 degrees C. Hence this media may be recommended for inoculum production for further studies.
可可毛色二孢，一种常见的茶树病原菌，在普通培养基中通常不产孢或产孢不良，这使得孢子生产困难。本研究评估了培养基、碳源、氮源、温度、pH值和光照对菌丝生长和产孢的影响。在测试的几种碳源中，发现葡萄糖和蔗糖对生长更有利。在测试的无机氮源中，添加硝酸钾的培养基生长最佳，而在测试的有机氮源中，蛋白胨的生长最佳。发现添加茶树根提取物的马铃薯葡萄糖琼脂培养基最适合可可毛色二孢的菌丝生长和产孢。该真菌在4至36摄氏度的温度范围内生长，最适生长温度为28摄氏度，在40摄氏度时不生长。不同光照周期对可可毛色二孢的生长没有显著影响，但光照可促进产孢。该真菌在pH值3.0至8.0的条件下生长，最适生长pH值为6.0。添加茶树根提取物、pH值为6.0的马铃薯葡萄糖琼脂培养基最适合在28摄氏度下生产可可毛色二孢的分生孢子。因此，这种培养基可推荐用于接种物生产以供进一步研究。
