Marinkovic S, Jahreis G P, Wong G G, Baumann H
Department of Molecular and Cellular Biology, Roswell Park Memorial Institute, Buffalo, NY 14263.
J Immunol. 1989 Feb 1;142(3):808-12.
Human rIL-6, produced either in COS cells or Escherichia coli, similarly stimulates the production of acute phase plasma proteins in cultured human and rat hepatoma cells. This anabolic effect in hepatoma cells suggested a potential in vivo role of the cytokine in mediating the hepatic response to inflammation. Injection of IL-6 into adult male rats elicited a cytokine-specific change in the liver expression of acute phase proteins. As predicted from in vitro studies, glucocorticoids were needed to achieve a maximal IL-6 response in vivo. Optimal conditions were found to be two i.p. injections of 35 to 120 micrograms IL-6 and 65 micrograms dexamethasone per kg body weight administered at 12-h intervals. Within 24 h, the plasma concentrations for alpha 2-macroglobulin, fibrinogen, thiostatin, and hemopexin were increased to levels approximating those observed in acute phase animals. These results support the notion that direct interaction of IL-6 with the liver is an essential part in initiating the hepatic acute phase reaction.
在COS细胞或大肠杆菌中产生的人重组白细胞介素-6（rIL-6）同样能刺激培养的人及大鼠肝癌细胞中急性期血浆蛋白的产生。肝癌细胞中的这种合成代谢作用提示细胞因子在介导肝脏对炎症反应中可能具有体内作用。向成年雄性大鼠注射白细胞介素-6会引起急性期蛋白肝脏表达的细胞因子特异性变化。正如体外研究所预测的，在体内需要糖皮质激素来实现白细胞介素-6的最大反应。发现最佳条件是每千克体重腹腔注射35至120微克白细胞介素-6和65微克地塞米松，间隔12小时给药。在24小时内，α2-巨球蛋白、纤维蛋白原、硫抑素和血红素结合蛋白的血浆浓度增加到接近急性期动物中观察到的水平。这些结果支持了白细胞介素-6与肝脏直接相互作用是启动肝脏急性期反应的重要部分这一观点。
