Wu Jianzhong, Chen Lin, Zhou Wenbin, Liu Xiaoan, Chen Xin, Miao Dengshun
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2013 Dec;27(12):1487-91.
To investigate the expression and prognostic value of vascular endothelial growth factor receptor 3 (VEGFR-3) in human breast cancer.
A tissue microarray containing cores was constructed from 173 female patients with primary breast cancers (experimental group) and 19 female patients with benign breast lesions (control group) between March 2004 and June 2007. HE staining was performed to determine the quality of tissue microarray and immunohistochemistry staining was used to detect VEGFR-3 expression in the tissue microarray. Statistical analysis was performed to show the association between VEGFR-3 expression and clinicopathologic parameters.
Histological examination showed that the tissue microarray had good quality, which could represent the histological characteristics of breast cancer and benign breast lesions. VEGFR-3 was expressed in 55.5% (96/173) breast cancer patients of experimental group, while the expression was negative in lesions of control group. VEGFR-3 expression rate had no significant relation with age, estrogen receptor, and progesterone receptor (P > 0.05). VEGFR-3 expression rate correlated positively with tumor size and pathological staging compared to VEGFR-3 negative tumors (P < 0.05). The positive expression rate of VEGFR-3 in breast cancer with lymph node metastasis was significantly higher than that without lymph node metastasis (P < 0.05). Furthermore, VEGFR-3 positive staining correlated with human EGF receptor 2 expression (P < 0.05).
The expressions of VEGFR-3 in human breast cancer have close relations with lymph node metastasis. Thus, VEGFR-3 may be a potential prognostic indicator and a new anti-cancer target for breast cancer.
探讨血管内皮生长因子受体3（VEGFR - 3）在人乳腺癌中的表达及预后价值。
2004年3月至2007年6月间，从173例原发性乳腺癌女性患者（实验组）和19例乳腺良性病变女性患者（对照组）构建含组织芯的组织芯片。进行苏木精 - 伊红（HE）染色以确定组织芯片质量，采用免疫组织化学染色检测组织芯片中VEGFR - 3的表达。进行统计学分析以显示VEGFR - 3表达与临床病理参数之间的关联。
组织学检查显示组织芯片质量良好，能够代表乳腺癌和乳腺良性病变的组织学特征。实验组173例乳腺癌患者中55.5%（96/173）表达VEGFR - 3，而对照组病变中表达为阴性。VEGFR - 3表达率与年龄、雌激素受体和孕激素受体无显著关系（P>0.05）。与VEGFR - 3阴性肿瘤相比，VEGFR - 3表达率与肿瘤大小和病理分期呈正相关（P<0.05）。有淋巴结转移的乳腺癌中VEGFR - 3阳性表达率显著高于无淋巴结转移者（P<0.05）。此外，VEGFR - 3阳性染色与人类表皮生长因子受体2表达相关（P<0.05）。结论：VEGFR - 3在人乳腺癌中的表达与淋巴结转移密切相关。因此，VEGFR - 3可能是一种潜在的预后指标和乳腺癌新的抗癌靶点。
