Nascimento Aline R, Pimenta Maristela T, Lucas Thais F G, Porto Catarina S, Lazari Maria F M
Ital J Anat Embryol. 2013;118(1 Suppl):26-8.
Immature Sertoli cells proliferate and several factors affect their number, including the follicle stimulating hormone (FSH), testosterone, estradiol and several paracrine growth factors. Using a primary culture of Sertoli cells isolated from 15-day old Wistar rats we have shown that relaxin stimulates Sertoli cell proliferation through the activation of MEK/ERK1/2 and PI3K/AKT pathways. In contrast, FSH inhibited both ERK1/2 and AKT phosphorylation. Furthermore, FSH strongly increased cAMP production, whereas relaxin inhibited basal cAMP production. Our results indicate that in rat Sertoli cells from 15-day old rats relaxin and FSH affect the same signaling pathways in opposite directions. Interplay between both hormones may be important to control the proliferation and differentiation of Sertoli cells.
未成熟的支持细胞会增殖，多种因素会影响其数量，包括促卵泡激素（FSH）、睾酮、雌二醇和几种旁分泌生长因子。我们利用从15日龄Wistar大鼠分离的支持细胞原代培养物，证明松弛素通过激活MEK/ERK1/2和PI3K/AKT信号通路来刺激支持细胞增殖。相反，FSH抑制ERK1/2和AKT的磷酸化。此外，FSH强烈增加cAMP的产生，而松弛素抑制基础cAMP的产生。我们的结果表明，在15日龄大鼠的支持细胞中，松弛素和FSH以相反的方向影响相同的信号通路。两种激素之间的相互作用可能对控制支持细胞的增殖和分化很重要。
