花生果皮中白藜芦醇含量的测定及其与碳水化合物代谢酶体外抑制活性的关系。

Estimation of resveratrol content in peanut pericarp and its relation to the in vitro inhibitory activity on carbohydrate metabolizing enzymes.

作者信息

Hetta M H, Aly H F, All N W

出版信息

Pharmazie. 2014 Feb;69(2):92-5.

Abstract

The aim of this work was the estimation of resveratrol content in two successive extracts (EtOAc and MeOH) of peanuts (Arachis hypogaea L.) pericarp of Egypt, by TLC and HPLC methods. Results showed the presence of 3.0 and 0.5 microg/mL resveratrol in EtOAc and MeOH extracts respectively. The in vitro carbohydrate hydrolyzing enzyme inhibition activity showed higher percentage of inhibition of alpha-amylase, alpha-glucosidase and beta-galactosidase with EtOAc (4.32, 5.93 and 13.7%) than with MeOH extract (3.9, 4.9 and 14.1%) but lower than the standard resveratrol concentration (5.18, 5.94 and 13.26%) and the reference acarbose (5.88, 5.9 and 13.0%). It could be concluded that the content of resveratrol in peanut pericarp is related to the percentage of inhibition activity of carbohydrate hydrolyzing enzymes. These results strongly reflect the benefit of using peanut pericarp, the waste product, as a natural antidiabetic agent.

摘要

本研究旨在通过薄层色谱法（TLC）和高效液相色谱法（HPLC）测定埃及花生（Arachis hypogaea L.）果皮连续两次提取物（乙酸乙酯提取物和甲醇提取物）中的白藜芦醇含量。结果显示，乙酸乙酯提取物和甲醇提取物中白藜芦醇的含量分别为3.0和0.5微克/毫升。体外碳水化合物水解酶抑制活性表明，乙酸乙酯提取物对α-淀粉酶、α-葡萄糖苷酶和β-半乳糖苷酶的抑制百分比（分别为4.32%、5.93%和13.7%）高于甲醇提取物（分别为3.9%、4.9%和14.1%），但低于标准白藜芦醇浓度（分别为5.18%、5.94%和13.26%）以及参比药物阿卡波糖（分别为5.88%、5.9%和13.0%）。可以得出结论，花生果皮中白藜芦醇的含量与碳水化合物水解酶的抑制活性百分比相关。这些结果有力地表明了将花生果皮这种废弃物用作天然抗糖尿病药物的益处。