Chemical Sciences Division, Oak Ridge National Laboratory , Oak Ridge, Tennessee, 37831, United States.
J Agric Food Chem. 2014 Mar 26;62(12):2595-604. doi: 10.1021/jf4055566. Epub 2014 Mar 18.
The development of deuterated biomass is essential for effective neutron scattering studies on biomass, which can provide key insights into the complex biomass conversion processes. A method for optimized production of deuterated annual ryegrass (Lolium multiflorum) was developed by growing the plants in 50% D2O in perfused hydroponic chambers. Deuterium incorporation of 36.9% was found in the annual rye grown in 50% D2O. Further, deuterium incorporation of 60% was achieved by germinating the rye seedlings in H2O and growing in 50% D2O inside the perfusion chambers. The characteristics related to enzymatic hydrolysis such as biomass composition, degree of polymerization, and cellulose crystallinity were compared with its control protiated counterpart. The cellulose molecular weight indicated slight variation while hemicellulose molecular weights and cellulose crystallinity remain unaffected with the deuteration.
发展氘代生物质对于生物质的有效中子散射研究至关重要，这可以为复杂的生物质转化过程提供关键的见解。通过在灌注水培室中用 50% D2O 培养植物，开发了一种优化生产氘代多年生黑麦草（Lolium multiflorum）的方法。在 50% D2O 中生长的多年生黑麦草的氘掺入率为 36.9%。此外，通过在 H2O 中发芽黑麦草幼苗并在灌注室内用 50% D2O 生长，实现了 60%的氘掺入。与对照质子化物相比，对与酶水解相关的特性（如生物质组成、聚合度和纤维素结晶度）进行了比较。纤维素分子量略有变化，而半纤维素分子量和纤维素结晶度不受氘代的影响。
