Romano G J, Harlan R E, Shivers B D, Howells R D, Pfaff D W
Rockefeller University, New York, New York 10021.
Mol Endocrinol. 1988 Dec;2(12):1320-8. doi: 10.1210/mend-2-12-1320.
The effects of estrogen on proenkephalin (PE) gene expression were measured in neurons of the ventromedial hypothalamus. Slot blot hybridization analysis indicates that the levels of PE mRNA in the ventromedial hypothalamus of ovariectomized rats increase 3.1-fold after 2 weeks of estrogen replacement. In situ hybridization reveals that the estrogen-inducible enkephalinergic neurons are located in the ventrolateral aspect of the ventromedial nucleus, a subnucleus known to contain many estrogen-concentrating neurons. The increase in PE mRNA levels is due to both a 63% increase in the number of detectable PE mRNA-containing neurons and a 2.0-fold increase in the levels of PE mRNA per enkephalinergic neuron (1.63 x 2.0 = 3.3-fold overall induction). This estrogen-regulated enkephalinergic cell group may represent part of the neural network mediating estrogen's effects on reproductive behavior and/or other neuroendocrine processes.
研究了雌激素对腹内侧下丘脑神经元中前脑啡肽（PE）基因表达的影响。狭缝印迹杂交分析表明，去卵巢大鼠腹内侧下丘脑的PE mRNA水平在雌激素替代2周后增加了3.1倍。原位杂交显示，雌激素诱导的脑啡肽能神经元位于腹内侧核的腹外侧，该亚核已知含有许多雌激素浓缩神经元。PE mRNA水平的增加是由于可检测到的含PE mRNA神经元数量增加了63%，以及每个脑啡肽能神经元的PE mRNA水平增加了2.0倍（总体诱导倍数为1.63×2.0 = 3.3倍）。这个受雌激素调节的脑啡肽能细胞群可能代表了介导雌激素对生殖行为和/或其他神经内分泌过程影响的神经网络的一部分。
