Laurent-Huck F M, Stoeckel M E, Felix J M
Laboratoire de Physiologie Générale, URA 1446 CNRS, Strasbourg, France.
Brain Res Dev Brain Res. 1991 Sep 19;62(1):33-43. doi: 10.1016/0165-3806(91)90187-n.
In the rat hypothalamus, proenkephalin (PE) mRNA synthetizing cells were detected by in situ hybridization, using synthetic oligodeoxy-nucleotides, from embryonic day 14 (E14) in the presumptive anterior hypothalamic area (AHA) and preoptic part of the bed nucleus of the stria terminalis (BST), and from E18 in the developing median preoptic area, perifornical area, suprachiasmatic nucleus, dorsomedial and ventromedial hypothalamic nuclei. In the paraventricular nucleus, cells expressed PE gene in the late prenatal stages; both parvo- and magnocellular neurons synthetized PEmRNA in the early postnatal stages. Cells expressing PE gene were observed after birth in the lateral preoptic area, lateral hypothalamus, medial and lateral parts of the BST. PEmRNA was also found from E14 in the striatum, from E18 in the central and medial amygdaloid nuclei, the medial group of the thalamic nuclei, and postnatally in a second more anterior structure of the thalamus. In the hypothalamus, a clear similarity was observed between adult and developmental distributions of PE gene expressing cells. The early onset of PE gene expression in the developing rat diencephalon suggests an involvement of PE in developmental processes, such as cell proliferation and differentiation; the presence of PE during the perinatal period may also indicate the appearance of adult neural regulations.
在大鼠下丘脑，采用合成寡脱氧核苷酸原位杂交法，在胚胎第14天（E14）于下丘脑前区（AHA）和终纹床核视前部（BST）的假定区域检测到前脑啡肽原（PE）mRNA合成细胞，在胚胎第18天于发育中的视前正中区、穹窿周区、视交叉上核、下丘脑背内侧核和腹内侧核检测到该细胞。在室旁核中，细胞在产前后期表达PE基因；在出生后早期，小细胞和大细胞神经元均合成PE mRNA。出生后在视前外侧区、下丘脑外侧区、BST的内侧和外侧部分观察到表达PE基因的细胞。从E14开始在纹状体中发现PE mRNA，从E18开始在杏仁中央核和内侧核、丘脑内侧核群中发现，出生后在丘脑第二个更靠前的结构中发现。在下丘脑中，观察到PE基因表达细胞的成年分布与发育分布之间有明显相似性。发育中大鼠间脑PE基因表达的早期开始表明PE参与发育过程，如细胞增殖和分化；围产期PE的存在也可能表明成年神经调节的出现。
