Arbatsky Nikolay P, Shashkov Alexander S, Kirsheva Nadezhda A, Kondakova Anna N, Shaikhutdinova Rima Z, Ivanov Sergey A, Anisimov Andrey P, Knirel Yuriy A
N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.
State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russia.
Carbohydr Res. 2015 Feb 11;403:202-5. doi: 10.1016/j.carres.2014.02.020. Epub 2014 Feb 28.
O-Polysaccharide was isolated from the lipopolysaccharide of an entomopathogenic bacterium Photorhabdus temperata subsp. temperata XlNach(T). Sugar analysis after full acid hydrolysis of the polysaccharide revealed D-glucose, D-mannose, D-galactose, D-GalNAc, and a branched monosaccharide, 3,6-dideoxy-4-C-[(S)-1',2'-dihydroxyethyl]-D-xylo-hexose (Sug), which was isolated as a 1,2'-anhydro furanose derivative. The following structure of the polysaccharide was established by 1D and 2D 1H and 13C NMR spectroscopy:
从昆虫致病细菌嗜温光杆状菌嗜温亚种XlNach(T)的脂多糖中分离出O-多糖。多糖经完全酸水解后的糖分析显示含有D-葡萄糖、D-甘露糖、D-半乳糖、D-氨基半乳糖，以及一种分支单糖3,6-二脱氧-4-C-[(S)-1',2'-二羟基乙基]-D-木糖己糖（Sug），其以1,2'-脱水呋喃糖衍生物形式分离得到。通过一维和二维氢谱及碳谱核磁共振波谱确定了该多糖的以下结构：
