Nász I
Institute of Microbiology, Semmelweis University Medical School, Budapest, Hungary.
Virologie. 1988 Oct-Dec;39(4):267-80.
Three panels of MAbs were prepared against AV1, AV35, and BAV2 hexons, respectively. It was shown, that in all cases, antibodies are developed against the genus specific determinant of the adenovirus hexon. The other MAbs specified numerous identical or overlapping epitopes on the hexon types studied. The epitopes could be characterized as intertype-, intersubgenus-, subgenus- and type-specific ones beside the genus-specific determinant based on the RPs of the MAbs from indirect ELISA and passive HA results. The identical epitopes are present in more than one copy on the trimeric form of the hexon capsomer. The epitopes on the hexon molecule could be separated into three antigenic sites, of which one antigenic site is characterized by seven epitope clusters (antigenic site II). Monoclonal antibodies were able to precipitate different hexon types in gel diffusion tests by which the differentiation of the distinct epitopes seemed to be possible. With the help of monoclonal antibodies to AV1 hexon, 17 hours after the infection, hexons (or epitopes) were detected in the cytoplasm and in the nucleus of the infected cells showing different distribution patterns in indirect immunofluorescence assay.
分别制备了针对腺病毒1型（AV1）、腺病毒35型（AV35）和禽腺病毒2型（BAV2）六邻体的三组单克隆抗体（MAbs）。结果表明，在所有情况下，均产生了针对腺病毒六邻体属特异性决定簇的抗体。其他单克隆抗体在研究的六邻体类型上确定了许多相同或重叠的表位。根据间接酶联免疫吸附测定（ELISA）和被动血凝（HA）结果中单抗的反应模式（RPs），除了属特异性决定簇外，这些表位可被表征为型间、亚属间、亚属和型特异性表位。相同的表位以多于一个拷贝的形式存在于六邻体壳粒的三聚体形式上。六邻体分子上的表位可分为三个抗原位点，其中一个抗原位点由七个表位簇组成（抗原位点II）。单克隆抗体能够在凝胶扩散试验中沉淀不同的六邻体类型，通过这种方法似乎可以区分不同的表位。借助针对AV1六邻体的单克隆抗体，在感染后17小时，在感染细胞的细胞质和细胞核中检测到六邻体（或表位），在间接免疫荧光试验中显示出不同的分布模式。
