Adám E, Nász I, Lengyel A
Institute of Microbiology, Semmelweis Medical University, Budapest, Hungary.
Arch Virol. 1996;141(10):1891-907. doi: 10.1007/BF01718202.
For the investigation of epitope composition of different adenovirus hexon types sixty-one mouse ascitic fluids containing monoclonal antibodies (MAbs) developed in three different panels were used. The distinction and marking of the different epitopes recognized by the MAbs were carried out by the determination of the composite cross-reactivity pattern, the titer and the correlation coefficient of all the 61 MAbs with 21 different hexon types representing all the six human subgenera, as well as different bovine and simian adenoviruses. The distinct epitopes were marked by two numbers referring the homologous hexon type to which the MAbs were directed and the serial number of the epitope specified by the different members of the given panel of the MAbs. The three panels of MAbs recognized 22 epitopes on the 21 hexon types among them a genus and three type specific ones and 18 different bi- and multilateral intertype (IT) specific epitopes that grouped adenoviruses within the genus, independently from the subgenus they belong to. Considering that the type specific epitope could be present only on the homologous hexon type, the largest number of the different epitopes distinguishable by the MAbs used could be 20 on the homologous hexon and 19 on the heterologous ones. It was found that the total number of IT specific epitopes on the hexons varied between 2 and 18. The distribution of the distinct specific epitopes on the different hexon types was different, as expected. The antigenic structure of the individual hexon types were characterized by the determination of their IT specific epitope spectrum. By pairwise analysis ten human hexon types formed three epitope clusters (types 4 and 19; types 8, 9, 9/13 and 10; as well as all types of subgenus C) showing identical epitope spectra. No clustering was found with human type 7, 12, 13, 18, 26, 27, 35 and 41, as well as with bovine and simian adenovirus hexons studied. However, they displayed a closer or looser antigenic relationship among each other and to members of the epitope clusters. The degree of antigenic relationship could be expressed by the similarity/dissimilarity percentage calculated from the number of the identical and different epitopes present on any two given hexon types.
为研究不同腺病毒六邻体类型的表位组成，使用了在三个不同组中产生的61种含有单克隆抗体（MAb）的小鼠腹水。通过测定61种MAb与代表所有六个人类亚属以及不同牛和猴腺病毒的21种不同六邻体类型的复合交叉反应模式、效价和相关系数，对MAb识别的不同表位进行区分和标记。不同的表位用两个数字标记，第一个数字表示MAb所针对的同源六邻体类型，第二个数字表示给定MAb组中不同成员指定的表位序列号。这三组MAb在21种六邻体类型上识别出22个表位，其中1个属特异性和3个型特异性表位，以及18个不同的双特异性和多特异性型间（IT）表位，这些表位将腺病毒属内的病毒分组，而不考虑它们所属的亚属。考虑到型特异性表位仅存在于同源六邻体类型上，所使用的MAb可区分的不同表位的最大数量在同源六邻体上为20个，在异源六邻体上为19个。发现六邻体上IT特异性表位的总数在2到18之间变化。不同六邻体类型上不同特异性表位的分布如预期的那样不同。通过测定其IT特异性表位谱来表征各个六邻体类型的抗原结构。通过成对分析，十种人类六邻体类型形成了三个表位簇（4型和19型；8型、9型、9/13型和10型；以及C亚属的所有类型），显示出相同的表位谱。在研究的人类7型、12型、13型、18型、26型、27型、35型和41型以及牛和猴腺病毒六邻体中未发现聚类。然而，它们彼此之间以及与表位簇成员之间显示出较近或较远的抗原关系。抗原关系的程度可以通过根据任意两种给定六邻体类型上相同和不同表位的数量计算出的相似性/不相似性百分比来表示。
