Kıter Esat, Er Ertan, Sen Türk Nilay, Oto Murat, Akkaya Semih
Department of Orthopedics and Traumatology, Pamukkale University Faculty of Medicine, Denizli, Turkey.
Department of Orthopedics and Traumatology, Acıpayam State Hospital, Denizli, Turkey.
Acta Orthop Traumatol Turc. 2014;48(1):86-91. doi: 10.3944/AOTT.2014.3161.
The aim of this study was to investigate the influence of the antiproliferative agent 5-Fluorouracil (5FU) over inhibition of spinal bone formation in an experimental rat model.
The study included 30 6-month-old Sprague-Dawley rats divided into 3 groups. Aggressive periosteal denuding was performed by scalpel to induce punctuate bleeding in the posterior elements of the L4-L5 vertebrae in the spontaneous fusion model. Spinous processes were fixated by wires and no graft substitute was applied. Adcon-L was applied in Group 1, single-touch technique 5FU in Group 2. Group 3 was the control group. Rats were sacrificed at 4 weeks and specimens acquired for histological examination.
5FU substantially inhibited fibroblast and inflammatory cell densities as well as bone formation compared to the control group. 5FU was considerably superior to Adcon-L with regard to inhibition of bone formation and inflammatory cells (p=0.0001).
5FU can inhibit fusion, fibrosis and unwanted scar tissue in spinal surgery. We believe that after further studies on its local delivery dose, it can be used in humans for inhibition of unintended fusion.
本研究旨在探讨抗增殖剂5-氟尿嘧啶（5FU）对实验性大鼠模型中脊柱骨形成抑制的影响。
该研究纳入30只6月龄的Sprague-Dawley大鼠，分为3组。在自发融合模型中，用手术刀进行侵袭性骨膜剥脱，以诱导L4-L5椎体后部结构出现点状出血。用钢丝固定棘突，不应用移植替代物。第1组应用Adcon-L，第2组应用单次接触技术的5FU。第3组为对照组。4周后处死大鼠，获取标本进行组织学检查。
与对照组相比，5FU显著抑制成纤维细胞和炎性细胞密度以及骨形成。在抑制骨形成和炎性细胞方面，5FU明显优于Adcon-L（p=0.0001）。
5FU可抑制脊柱手术中的融合、纤维化和不必要的瘢痕组织。我们认为，在对其局部给药剂量进行进一步研究后，它可用于人体以抑制意外融合。
